摘要
目的建立低共熔溶剂(deep eutectic solvent,DES)-超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)快速检测肉制品、水产品中3种非法添加工业染料(刚果红、酸性金黄、红2G)的分析方法。方法样品经水稀释,微量DES分散萃取,乙腈复溶。三重四极杆电喷雾离子源电离-多反应监测(multiple reaction monitoring,MRM)-负离子监测模式检测,外标法定量。结果刚果红和红2G在0.2~20.0μg/kg范围内、酸性金黄在0.02~2.00μg/kg范围内线性关系良好,相关系数均大于0.99。方法的检出限分别为0.12、0.26、0.01μg/kg,回收率为93.25%~101.36%,相对标准偏差为1.27%~4.06%。结论低共熔溶剂对刚果红、酸性金黄、红2G的萃取效果佳,可以解决传统操作步骤烦琐的问题。本方法快速、简便、精准,适用于肉制品、水产品中3种非法添加工业染料刚果红、酸性金黄、红2G的检测。
Objective To establish a rapid method for determination of 3 kinds of illegally added industrial dyes(Congo red,methaniol yellow,red 2G)in meat and aquatic product by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)with deep euectic solvent(DES).Methods The samples were dissolved by water,dispersion extracted by microscale deep euectic solvent,and dissoluted by acetonitrile.Triple quadrupole electrospray ion source ionization-multiple reaction monitoring(MRM)-anion monitoring mode was used for detection,external standard method was used for quantitative analysis.Results The linear relationships of Congo red,red 2G in the ranges of 0.2-20.0μg/kg and metanil yellow in the ranges of 0.02-2.00μg/kg were good,respectively,and the correlation coefficients were greater than 0.99.The limits of detection of the method were 0.12,0.26 and 0.01μg/kg,respectively.The recovery rates were 93.25%-101.36%,and relative standard deviations were 1.27%-4.06%.Conclusion DES has a good extraction effect of Congo red,red 2G and metanil yellow,which can solve the problem of complicated tradittional operation steps.This method is rapid,simple and accurate,and is suitable for determination of 3 kinds of illegally added industrial dyes in meat and aquatic products.
作者
周佳
刘子溱
王丽君
贾红芳
文树新
朱书强
ZHOU Jia;LIU Zi-Zhen;WANG Li-Jun;JIA Hong-Fang;WEN Shu-Xin;ZHU Shu-Qiang(Gansu Food Inspection and Research Institute,Lanzhou 730300,China;Gansu Engineering Research Center of Nutrition and Safety Evaluation of Geographical Indication Products,Lanzhou 730300,China;Gansu Province Product Quality Supervision and Inspection Research Institute,Lanzhou 730030,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2022年第5期1538-1544,共7页
Journal of Food Safety and Quality
基金
甘肃省科技计划重点研发项目(18YF1FA048)
甘肃省市场监督管理局科技计划项目(SSCJG-SP-201915)
甘肃省知识产权计划项目(21ZSCQ072)。
关键词
低共熔溶剂
非法添加工业染料
绿色溶剂
超高效液相色谱-串联质谱法
deep eutectic solvent
illegally added industrial dyes
green solvent
ultra performance liquid chromatography-tandem mass spectrometry
