摘要
以温室黄瓜连作4 a(T4)和11 a(T11)土壤为试材,通过分别添加腐植酸300倍液(300T4、300T11)和腐植酸600倍液(600T4、600T11)的盆栽试验,探究腐植酸对不同连作年限土壤养分和微生物群落的影响。结果表明,施入腐植酸后,不同连作年限土壤速效磷含量、速效钾含量、全氮含量和有机质含量均升高,pH值降低;与不添加腐植酸的T4处理相比,连作4 a土壤增施腐植酸后,只有600T4处理细菌的Shannon指数显著降低,降低了8.38%;腐植酸对不同连作年限土壤真菌群落α多样性无显著影响。在门分类水平上,连作4 a的土壤增施腐植酸后,600T4处理细菌的优势菌种占总数的比值高于T4和300T4处理,且分别高2.00%和1.96%;600T11处理优势菌种所占比值在连作11 a的处理中同样为最高。连作4 a土壤添加腐植酸后,子囊菌门(Ascomycota)的相对丰度升高,担子菌门(Basidiomycota)的相对丰度降低;连作11 a土壤施入腐植酸后,300T11和600T11处理子囊菌门相对丰度分别较不添加腐植酸的T11降低5.61%和2.22%,300T11处理担子菌门、接合菌门(Zygomycota)和被孢霉门(Mortierellomycota)的相对丰度最高,600T11处理壶菌门(Chytridiomycota)的相对丰度高于300T11和T11处理。在属分类水平上,腐植酸300倍液显著提高了细菌中Chryseolinea和RB41的相对丰度,腐植酸600倍液则显著提高了假单胞菌属(Pseudomonas)和Altereythrobacter的相对丰度;连作11 a的处理中,腐植酸300倍液处理被孢霉属(Mortierella)和鬼伞属(Coprinus)的相对丰度较高,腐植酸600倍液处理假霉样真菌属(Pseudallescheria)的相对丰度最高。冗余分析(Redundancy analysis,RDA)结果表明,腐植酸改变了连作11 a土壤细菌和真菌的群落结构。
With 4 years(T4)and 11 years(T11)soil of cucumber continuous cropping in greenhouse as test materials,the effects of humic acid on soil nutrients and microbial community of continuous cropping for different years were studied by adding 300 times solution of humic acid(300T4,300T11)and 600times solution of humic acid(600T4,600T11)respectively.The results showed that after applying humic acid,the content of available phosphorus,available potassium,total nitrogen and organic matter increased and the pH value decreased in different continuous cropping years.Compared with T4 treatment,the Shannon index of bacteria in 600T4 treatment decreased significantly by 8.38%.Humic acid had no significant effect onαdiversity of soil fungal communities in different years.At the taxonomic level of phylum,the ratio of dominant bacteria in 600T4 treatment was higher than that in T4 and 300T4treatments,and was 2.00%and 1.96%higher respectively;The ratio of dominant strains in 600T11treatment was also the highest in 11 years continuous cropping treatments.After adding humic acid in soil with continuous cropping for 4 years,the relative abundance of Ascomycota increased and Basidiomycota decreased.After adding humic acid in soil with continuous cropping for 11 years,the relative abundance of Ascomycota in 300T11 and 600T11 treatments decreased by 5.61%and 2.22%respectively compared with T11.The relative abundance of Basidiomycota,Zygomycota and Mortierellomycota in 300T11treatment was the highest,and the relative abundance of Chytridiomycota in 600T11 treatment was higher than that in 300T11 and T11 treatments.At the genus classification level,300 fold solution of humic acid significantly increased the relative abundance of Chryseolinea and RB41 in bacteria;600 fold solution of humic acid significantly increased the relative abundance of Pseudomonas and Altereythrobacter;In the treatments of continuous cropping for 11 years,the relative abundance of Mortierella and Coprinus were higher in 300 fold solution of humic acid,and the relative abundance of Pseudallescheria treated with humic acid 600 fold solution of humic acid was the highest.Redundancy analysis(RDA)showed that humic acid changed the community structure of soil bacteria and fungi after continuous cropping for 11 years.
作者
孙世君
刘琦
叶英杰
魏娜
郝水源
SUN Shijun;LIU Qi;YE Yingjie;WEI Na;HAO Shuiyuan(Department of Agronomy,Hetao College,Bayannur 015000,China;Tongliao Agriculture and Animal Husbandry Research Institute,Tongliao 028000,China;Bayannur Agricultural and Animal Husbandry Technology Extension Center,Bayannur 015000,China)
出处
《河南农业科学》
北大核心
2022年第2期65-74,共10页
Journal of Henan Agricultural Sciences
基金
内蒙古自治区自然科学基金项目(2021BS03009)
内蒙古自治区科技计划项目(19041201011)。
关键词
腐植酸
黄瓜
连作年限
土壤养分
微生物
Humic acid
Cucumber
Continuous cropping years
Soil nutrient
Microorganism
