摘要
目的:探讨前列腺癌(PCa)组织中微小核糖核酸-183-5p(miR-183-5p)表达水平及其诊断价值。方法:选择我院2018年9月至2021年5月收治的PCa患者117例作为PCa组,同期收治的良性前列腺增生(BPH)患者65例为BPH组。采用实时逆转录聚合酶链反应(qRT-PCR)检测两组样本组织中miR-183-5p表达水平,分析其与PCa病理特征的关系;绘制受试者工作特征(ROC)曲线分析miR-183-5p诊断PCa的曲线下面积(AUC),利用Kappa检验分析miR-183-5p与病理诊断PCa的一致性。结果:PCa组miR-183-5p表达量高于BPH组(P<0.05)。miR-183-5p高表达组血清前列腺特异性抗原(PSA)水平≥20 ng·ml^(-1)、Gleason评分≥7分、肿瘤低分化、肿瘤分期Ⅲ期、肿瘤直径≥3 cm、血管间隙浸润、肿瘤转移者占比均高于miR-183-5p低表达组(均P<0.05)。miR-183-5p表达量诊断PCa的AUC为0.898(95%CI为0.851~0.945,P<0.05)。Kappa检验显示miR-183-5p表达量与病理诊断PCa具有高度一致性(Kappa值=0.708,P<0.05)。结论:PCa患者癌组织中的miR-183-5p表达量上调,其表达与血清PSA水平、Gleason评分、肿瘤分化程度、肿瘤分期、肿瘤直径、血管间隙浸润、肿瘤转移存在关联,对PCa具有一定诊断价值。
Objective:To explore the expression level of microribonucleic acid-183-5p(miR-183-5p)in prostate cancer(PCa)tissue and its diagnostic value.Methods:A total of 117 PCa patients admitted to our hospital from September 2018 to May 2021 were included as PCa group,and 65 patients with benign prostatic hyperplasia(BPH)admitted during the same period were selected as BPH group.Quantitative real-time reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the expression level of miR-183-5p in the tissues of the two groups,and its relationship with the pathological characteristics of PCa was analyzed.Receiver operating characteristic(ROC)curve was drawn to analyze the area under the curve(AUC)of miR-183-5p in the diagnosis of prostate cancer.Kappa test was employed to analyze the consistency of miR-183-5p with pathological diagnosis of PCa.Results:The expression of miR-183-5p in PCa group was higher than that in BPH group(P<0.05).The levels of serum prostate specific antigen(PSA)in miR-183-5p high expression group were≥20 ng·ml^(-1),Gleason score was≥7,and the tumor was poorly differentiated and in stageⅢwith diameter≥3 cm.The proportion of vascular space infiltration and tumor metastasis was lower than miR-183-5p low expression group(P<0.05).The AUC of miR-183-5p expression to diagnose PCa was 0.898(95%CI 0.851-0.945,P<0.05).Kappa test showed that the expression of miR-183-5p was highly consistent with the pathological diagnosis of PCa(Kappa value=0.708,P<0.05).Conclusion:The expression of miR-183-5p is up-regulated in PCa patients,and its expression is correlated with serum PSA level,Gleason score,degree of differentiation,tumor stage and diameter,vascular space invasion and tumor metastasis,which has certain diagnostic value for PCa.
作者
吴继宏
梁泰生
高宏君
罗向东
吴刚
WU Jihong;LIANG Taisheng;GAO Hongjun;LUO Xiangdong;WU Gang(Department of Urology, Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine, Nanning 530000, China)
出处
《东南大学学报（医学版）》
CAS
2022年第2期231-236,共6页
Journal of Southeast University(Medical Science Edition)
基金
广西自然科学基金资助项目(2017GXNSFAA198080)。
