摘要
[目的]探究阴虚质与平和质原发性高血压(essential hypertension,EH)患者血管紧张素转化酶(angiotensin-converting enzyme,ACE)、血管紧张素Ⅱ-1型受体(angiotensinⅡ-type 1 receptor,AT1R)、血管紧张素Ⅱ-2型受体(angiotensinⅡ-type 2 receptor,AT2R)、Mas受体(Mas receptor,MasR)4个基因下的13个单核苷酸多态性(single nucleotide polymorphism,SNP)的位点差异。[方法]纳入符合标准的70例阴虚质及30例平和质EH患者,采集静脉血,对ACE、AT1R、AT2R、MasR 4个基因下13个SNP位点进行测序分型。[结果] 13个SNP位点均符合哈迪-温伯格平衡定律(P>0.05),样本代表性良好。AT1R基因rs5182基因型CC、CT和TT在平和质EH患者中的频率分布分别为16.7%、36.7%和46.7%,在阴虚质EH患者中频率分布分别为2.9%、58.6%和38.6%,两组之间差异有统计学意义(P<0.05);基因型CC与CT+TT在平和质EH患者中频率分布分别为16.7%和83.3%,在阴虚质EH患者中频率分布分别为2.9%和97.1%,两组之间差异有统计学意义(P<0.05)。[结论] EH患者AT1R基因rs5182位点的多态性可能与阴虚质存在关联,T等位基因可能是EH患者阴虚质的易感基因。
[Objective] To explore the differences between the two corporeities from the genetic level by comparing the locus polymorphism of the 13 single nucleotide polymorphism(SNP) under four genes including angiotensin converting enzyme(ACE), angiotensin Ⅱ-type 1 receptor(AT1 R), angiotensin Ⅱ-type 2 receptor(AT2 R) and Mas receptor(MasR) in Yin deficiency constitution and normal constitution of essential hypertension(EH) patients. [Methods] Totally 70 EH patients with Yin deficiency and 30 EH patients with normal constitution were enrolled in the present study. Venous blood was collected and 13 SNPs under 4 genes including ACE, AT2 R, AT1 R and MasR were sequenced and typed. [Results] All 13 SNP loci conformed to Hardy-Weinberg equilibrium law(P >0.05), and the samples were well represented. The frequencies of CC, CT and TT genotypes of AT1 R rs5182 in EH patients with normal constitution were 16.7%, 36.7%and 46.7% respectively, meanwhile the frequencies in EH patients with Yin deficiency constitution were 2.9%, 58.6% and 38.6%, there was significant difference between the two groups(P <0.05). The frequency of genotype CC and CT +TT in EH patients with normal constitution was 16.7% and 83.3%, and the frequencies in EH patients with Yin deficiency were 2.9% and 97.1%, and there was significant difference between the two groups(P<0.05). [Conclusion] The polymorphism of rs5182 locus of AT1 R gene in EH patients may be associated with Yin deficiency constitution, and the T allele may be a susceptibility gene for Yin deficiency constitution in EH patients.
作者
丁磊
许月萍
陆海娟
沈翠珍
DING Lei;XU Yueping;LU Haijuan(Zhejiang Chinese Medical University,Hangzhou(310053),China;Jiaxing TCM Hospital)
出处
《浙江中医药大学学报》
CAS
2022年第2期133-137,共5页
Journal of Zhejiang Chinese Medical University
