摘要
目的探讨四神丸挥发油对溃疡性结肠炎小鼠滤泡辅助性T细胞(Tfh)亚群的调节作用。方法将BABL/c雄性小鼠随机分为正常组、模型组、四神丸挥发油组(27.71μL·kg^(-1))、二神丸挥发油组(19.45μL·kg^(-1))、五味子散挥发油组(8.26μL·kg^(-1))、美沙拉嗪组(300 mg·kg^(-1)),每组10只。采用3%葡聚糖硫酸钠(DSS)诱导溃疡性结肠炎小鼠模型,以自由饮水的方式持续造模7 d;自饮用DSS第1天起,各组按相应剂量灌胃给药,每日1次,连续10 d。每日称小鼠体质量,观察其一般症状并计算疾病活动指数(DAI)评分;测定小鼠结肠长度、质量及结肠质量指数、单位长度结肠质量;采用HE染色法观察结肠组织病理变化;采用流式细胞术检测外周血Tfh1、Tfh9、Tfh10、Tfh17、Tfh21及Tfr细胞水平。结果与模型组比较,四神丸挥发油组及五味子散挥发油组的小鼠体质量明显升高(P<0.05),DAI评分明显下降(P<0.05),结肠长度显著增加(P<0.01),结肠质量、结肠质量指数及单位长度结肠质量明显降低(P<0.05,P<0.01),结肠组织病理损伤明显改善,Tfh1、Tfh9、Tfh17、Tfh21细胞比例显著降低(P<0.01),Tfh10、Tfr细胞比例显著升高(P<0.01)。结论四神丸挥发油能够有效改善溃疡性结肠炎小鼠的症状,修复结肠黏膜损伤,可能与其调控Tfh细胞亚群间的平衡有关。
Objective To investigate the regulatory effects of volatile oil from Sishen Pills on follicular helper T cells(Tfh) subsets in mice with ulcerative colitis(UC).Methods BABL/c male mice were randomly divided into normal group,model group,Sishen Pills volatile oil group(27.71 μL·kg^(-1)),Ershen Pills volatile oil group(19.45 μL·kg^(-1)),Wuweizi Powder volatile oil group(8.26 μL·kg^(-1)),and Mesalazine group(300 mg·kg^(-1)),10 mice in each group. The model of UC was induced by 3% dextran sodium sulphate(DSS) and moulded continuously with free access to water for 7 days;From the first day of drinking DSS,each group was administered with the appropriate dose by gavage once daily for 10 days. The body mass of mice was weighed daily,and their general symptoms were observed and the disease activity index(DAI) score was calculated;the length and mass of mice’s colon and colon mass index were measured;the histopathological changes of colon were observed by HE staining^(-1)the levels of peripheral blood Tfh1,Tfh9,Tfh10,Tfh17,Tfh21 and Tfr cells were measured by flow cytometry(FCM).Results Compared with the model group,the mice body mass in the Sishen Pills volatile oil group and Wuweizi Powder volatile oil group was significantly increased(P<0.05), the DAI scores was significantly declined(P<0.05), the colon length was significantly increased(P<0.01), the colon mass, colon mass index and per unit length of colon mass were significantly decreased(P<0.05, P<0.01), the histopathological leison to the colon tissue was significantly improved,and the cell proportions of Tfh1,Tfh9,Tfh17 and Tfh21 were significantly reduced(P<0.01)and the cell proportions of Tfh10 and Tfr were significantly increased(P<0.01).Conclusion Sishen Pills volatile oil has significant effect in improving the symptoms and repairing injured colonic mucosa in UC mice,which may be related to its regulation of the balance between subpopulations of Tfh cells.
作者
王梦雪
蒋青青
黄佳琦
刘端勇
黄小英
赵海梅
WANG Mengxue;JIANG Qingqing;HUANG Jiaqi;LIU Duanyong;HUANG Xiaoying;ZHAO Haimei(Graduate School of Jiangxi University of Chinese Medicine,Nanchang 330004 Jiangxi,China;Science and Technology College of Jiangxi University of Traditional Chinese Medicine,Nanchang 330004 Jiangxi,China;Key Laboratory of Ministry of Education,Jiangxi University of Chinese Medicine,Nanchang 330004 Jiangxi,China;School of Traditional Chinese Medicine,Jiangxi University of Chinese Medicine,Nanchang 330004 Jiangxi,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2022年第3期293-299,共7页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家自然科学基金项目(82060838,82060799)
江西省自然科学基金项目(20202ACBL206026,20192ACB20015,20202ACBL206028,20181BAB205082)
江西省教育厅科技项目(GJJ196049,GJJ171536,GJJ170733)
江西中医药大学2021年国家级大学生创新创业训练计划项目(202110412007)。
关键词
四神丸
挥发油
溃疡性结肠炎
滤泡辅助性T细胞
小鼠
SishenPills
volatile oil
ulcerative colitis(UC)
follicular helper T cells(Tfh)
mice
