摘要
提取不同产地铁皮石斛醇提物,运用超高效液相色谱-串联四级杆-飞行时间质谱(UPLC-Q-TOF-MS)等技术对其进行分析。结合数据分析软件和本地数据库,对化学成分进行分析。结果:从醇提物中共鉴定了(-)-lirioresinol B、fructose-phenylalanine、香草酸、对羟基苯甲酸、石斛碱等32种成分,其中13种化合物为共有成分。同时对铁皮石斛中主要的两个成分——石斛酚和柚皮素的含量进行了定量分析,结果显示在不同产地铁皮石斛醇提物中这两种化合物的含量存在差异。石斛酚的含量范围为0.080 0~9.593 5 mg/g,柚皮素的含量范围为0.032 1~0.187 3 mg/g。结果证明石斛酚和柚皮素可以作为潜在的铁皮石斛质量评价指标。本次实验对全国主产区的铁皮石斛中化学成分进行了全面系统的分析,确证了前人的研究成功并进一步提出了两类小分子成分是成为完善现有铁皮石斛质量评价标准的潜在新指标,为完善铁皮石斛质量评价体系提供数据支撑。
The ethanol extracts of Dendrobium officinale from different habitats were analyzed by UPLC-Q-TOF-MS.Data analysis software combined with local database was used to analyse the chemical composition.A total of 32 compounds including(-)-lirioresinol B,fructose phenylalanine,vanillic acid,p-hydroxybenzoic acid and dendrobine were identified,among which 13 compounds were common components.The contents of gigantol and naringin,the two main components of D.officinale,were quantitatively analyzed.The results showed that the contents of the two compounds were different in the ethanol extracts of D.officinale from different habitats.The content of dendrobiol ranged from 0.080 0 to 9.593 5 mg/g,and the content of naringin ranged from 0.032 1 to 0.187 3 mg/g.The results showed that these two compounds could be used as potential quality evaluation indexes of D.officinale.This experiment conducted a comprehensive and systematic analysis of the chemical components in D.officinale from the main production areas in China.The results confirmed the accuracy of previous studies,and further proposed that the two types of compounds are potential new indicators to improve the existing quality evaluation standards of D.officinale.
作者
陈定康
李芝兰
杨柳
胡江苗
CHEN Ding-kang;LI Zhi-lan;YANG Liu;HU Jiang-miao(Kunming Institute of Botany,China Academy of Sciences;R&D Center of Dr.Plant,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650201,China;University of Chinese Academy of Science,Beijing 100049,China)
出处
《天然产物研究与开发》
CAS
CSCD
2022年第4期614-622,共9页
Natural Product Research and Development
基金
北京植物医生生物科技有限公司企业委托项目(E0514252C1)。
