摘要
具核梭杆菌(Fusobacterium nucleatum,Fn)是一种口腔中常见的条件致病菌,已有研究发现该菌是结肠癌、乳腺癌及肺癌患者的主要共生微生物,可保护肿瘤细胞抵抗抗生素并促进其增殖及转移。本研究旨在探究L-3-苯乳酸对Fn的抑制效果及相关机制,为通过食用天然生物酸预防癌症研究提供方向。通过Fn生长曲线的测定,确定Fn最佳生长周期及苯乳酸对其最小抑菌质量浓度,探究在苯乳酸作用下的Fn细胞膜形态、完整性及受损程度等,确定苯乳酸抑制Fn的能力。结果表明:Fn的最佳生长周期为27 h;苯乳酸对Fn的最小抑菌质量浓度为0.50 mg/mL;0.50 mg/mL苯乳酸处理2 h,Fn的细胞膜通透性增加,菌体形态明显改变;苯乳酸对Fn形成的生物膜有破坏作用,且降低了Fn的黏附性。结论:苯乳酸具有破坏Fn细胞膜结构及抑制其形成生物膜的能力,可使其内容物外泄,胞内蛋白含量降低。
Fusobacterium nucleatum(Fn),a common conditioned oral pathogen,has been found to be able to protect colon,breast and lung cancer cells against antibiotics and promote their proliferation and metastasis.The purpose of this study was to explore the inhibitory effect and mechanism of L-3-phenyllactic acid on Fn to provide directions for cancer prevention through the consumption of natural biological acids.Based on its growth curve,the optimum growth period of Fn and the minimum inhibitory concentration(MIC)of phenyllactic acid against Fn were determined,and the changes in the morphology,integrity and damage degree of the Fn cell membrane under the action of phenyllactic acid were investigated for the purpose of evaluating the ability of phenyllactic acid to inhibit Fn.The results showed that the optimum growth period of Fn was 27 h,and that the MIC of phenyllactic acid on Fn was 0.50 mg/mL.Treatment with phenyllactic acid at the MIC for 2 h increased the cell membrane permeability and changed significantly the cell morphology of Fn.Phenyllactic acid could disrupt the biofilm formed by Fn and reduce Fn adhesion.This study confirmed that phenyllactic acid had the ability to destroy the cell membrane structure of Fn and inhibit its biofilm-forming ability,thereby leading to the leakage of cellular contents and a reduction in the intracellular protein content.
作者
孔祥丽
马岩石
吴昕雨
许晓曦
KONG Xiangli;MA Yanshi;WU Xinyu;XU Xiaoxi(Key Laboratory of Dairy Science,Ministry of Education,School of Food Science,Northeast Agricultural University,Harbin 150030,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第7期38-44,共7页
Food Science
关键词
苯乳酸
最小抑菌浓度
具核梭杆菌
细胞膜通透性
生物膜
phenyllatic acid
minimum inhibitory concentration
Fusobacterium nucleatum
cell membrane permeability
biofilm
