摘要
背景:研究证实牙本质颗粒具有良好的成骨潜能,其中未脱矿牙本质颗粒的空间维持作用好,但降解吸收缓慢,延缓了新骨生成速度;完全脱矿牙本质颗粒降解快,但支架作用不佳、新骨生成量有限。目的:评价部分脱矿牙本质颗粒作为骨移植材料的组织反应与成骨效果。方法:收集临床上拔除的废弃牙齿,去除牙釉质、牙骨质和牙髓,保留健康的牙本质,经过粉碎、筛选、煮沸、灭菌等处理制成直径0.5-1.0 mm的未脱矿牙本质颗粒;将牙本质颗粒先浸泡在2%硝酸中脱矿10 min,制成部分脱矿牙本质颗粒。取5只比格犬进行双侧上颌窦开窗提升骨缺损造模,随机分为2组,实验组植入部分脱矿异种牙本质颗粒,对照组植入未脱矿异种牙本质颗粒,术后3个月对植骨区进行大体观察、影像学检查和组织学测量分析。结果与结论:①大体观察:两组植骨区愈合良好,无炎症反应,实验组植骨区表面较平坦,可见少量残留牙本质颗粒包埋在骨基质中;对照组植骨区表面不平整,可见较多牙本质颗粒;②锥形束CT检查:实验组植骨区为低密度的阻射影,牙本质颗粒与周围组织边界不清,移植的牙本质颗粒总体积减小,牙本质颗粒间影像密度较高;对照组可见高密度的牙本质颗粒阻射影,与周围组织边界较明显,牙本质颗粒吸收程度较实验组轻;③组织学观察:两组植骨区均未见明显的炎症细胞浸润,实验组牙本质颗粒呈虫蚀状吸收,周围有新生骨组织围绕,新生编织骨较致密;对照组牙本质颗粒吸收程度较实验组轻,新骨生成率低于实验组(P<0.05);④结果表明:两种牙本质颗粒均可以诱导新骨形成,在相同时间内,部分脱矿牙本质颗粒的成骨效果优于未脱矿牙本质颗粒。
BACKGROUND:It has been confirmed that dentin particles obtain good osteogenic potential.The non-demineralized dentin particles maintained well in space,but their degradation and absorption were slow,which delayed the rate of new bone formation.The fully demineralized dentine particles degraded rapidly,but the effect of scaffolds was not good,and the amount of new bone formation was limited.OBJECTIVE:To evaluate the tissue reaction and osteogenic effect of partially demineralized dentin matrixs as bone graft materials.METHODS:The enamel,cementum,and pulp were removed from clinically collected teeth,and the healthy dentin was preserved.The undemineralized dentin particles of 0.5-1.0 mm in diameter were prepared by grinding,screening,boiling,and sterilization.The dentin particles were demineralized in 2%nitric acid for 10 minutes,then cleaned and disinfected to produce partially demineralized dentin particles.Five beagles were selected for bilateral maxillary sinus elevation and randomly divided into two groups.Sinus in the experimental group was filled with partially demineralized dentin particles,and that in the control group was filled with undemineralized dentin particles.Three months after bone grafting,specimens were analyzed by gross observation,X-ray imaging and histological examination.RESULTS AND CONCLUSION:(1)General observation:The bone graft area in the two groups healed well without inflammation.In the experimental group,the surface of the bone graft area was flat,with remaining dentin particles.In the control group,the surface of the bone graft area was uneven,with a large number of remaining dentin particles.(2)Cone beam CT examination:In the experimental group,low-density image was observed;the boundary between dentin particles and surrounding tissues was unclear;the total volume of dentin particles in the bone graft area was decreased;and the density of new bone between dentin particles was increased.In the control group,the high density dentin particle image and surrounding tissue boundary were obvious,and dentin absorption was less compared with the experimental group.(3)Histological analysis:There was no obvious inflammatory cell infiltration in the bone graft area of the two groups.In the experimental group,dentin particles were absorbed like insect erosion,surrounded by new bone tissue;newly-born braided bones were dense.In the control group,the absorption degree of dentin particles was less than that in the experimental group.The rate of new bone formation was lower in the control group than that in the experimental group(P<0.05).(4)The results show that both partially demineralized and undemineralized dentin particles could induce new bone formation.The osteogenic effect of partially demineralized dentin particles was better than that of undemineralized dentin particles.
作者
刘欢
李涵
马云豪
仲维剑
马国武
Liu Huan;Li Han;Ma Yunhao;Zhong Weijian;Ma Guowu(Stomatological Hospital of Stomatology College of Dalian Medical University,Dalian 116023,Liaoning Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第3期354-359,共6页
Chinese Journal of Tissue Engineering Research
基金
辽宁省自然科学基金(2015020313),项目负责人:仲维剑。
关键词
骨
部分脱矿
牙本质颗粒
骨再生
生物材料
骨移植材料
上颌窦提升
动物实验
bone
partial demineralization
dentin particles
bone regeneration
biomaterial
bone graft
maxillary sinus lift
animal experiment
