摘要
欧李富含钙素,营养丰富,且具有免疫功能,研究欧李多糖的制备、结构及免疫调节活性,可为欧李深加工提供基础。本文以欧李为原料,采用水提醇沉法提取欧李多糖(Cerasus humilis polysaccharide,CHP),利用响应面法优化提取工艺。对提取的欧李多糖用DEAE-52纤维素层析柱、G-100葡聚糖凝胶柱纯化。用高效液相色谱、凝胶渗透色谱和红外光谱对欧李多糖结构表征,并测定欧李多糖的免疫调节活性。结果表明,欧李多糖最佳提取工艺条件为提取温度79℃,提取时间2 h,液料比16∶1(mL/g)。在此条件下,欧李干粉多糖得率为(32.18±0.08)%。纯化后欧李多糖主要有CHPP-1和CHPP-2两个组分,CHPP-1、CHPP-2中多糖含量分别为99.16%和99.33%。CHPP-1的单糖组成及摩尔占比为阿拉伯糖∶半乳糖醛酸∶葡萄糖=51.4∶20.29∶17.36,分子量为47.26 kDa。CHPP-2的单糖组成及摩尔占比为阿拉伯糖∶半乳糖醛酸∶葡萄糖=41.81∶28.24∶11.68,分子量为22.94 kDa。两个组分均为吡喃环型多糖。CHPP-2可显著增强巨噬细胞增殖活性,有效刺激细胞释放NO和TNF-α、IL-6及IFN-β。欧李多糖含量丰富,且具有较强的免疫调节活性。
Cerasus humilis is rich in calcium,rich in nutrition,and has immune function.Research on the preparation,structure and immunoregulatory activity of C.humilis polysaccharide(CHP)can provide basis for further processing of C.humilis.In this paper,the CHP was extracted by water extraction and alcohol precipitation method using C.humilis as raw material,and the extraction process was optimized by response surface methodology.The CHP was purified by DEAE-52 cellulose chromatography column and G-100 Sephadex gel column.The structure of CHP was characterized by high performance liquid chromatography,gel permeation chromatography and infrared spectroscopy,and the immunoregulatory activity of CHP was determined.The results showed that the optimal extraction process conditions of CHP were as follows:extraction temperature 79℃,extraction time 2 h,liquid-solid ratio 16∶1(mL/g).Under these conditions,the polysaccharide yield of dried C.humilis powder was(32.18±0.08)%.There were CHPP-1 and CHPP-2 in the purified C.humilis polysaccharide.The content of CHPP-1 and CHPP-2 were 99.16%and 99.33%,respectively.The monosaccharide composition and molar ratio of CHPP-1 were arabinose∶galacturonic acid∶glucose=51.4∶20.29∶17.36,molecular weight was 47.26 kDa.The monosaccharide composition and molar ratio of CHPP-2 were arabinose∶galacturonic acid∶glucose=41.81∶28.24∶11.68,molecular weight was 22.94 kDa.Both components were pyranoid ring polysaccharides.CHPP-2 can significantly enhance the proliferation of macrophages and effectively stimulate the release of NO,TNF-α,IL-6 and IFN-β.The CHP is rich in content and has strong immunomodulatory activity.
作者
冯荦荦
王锐
董兆斌
廖玉美
王国良
张润光
张有林
FENG Luo-luo;WANG Rui;DONG Zhao-bin;LIAO Yu-mei;WANG Guo-liang;ZHANG Run-guang;ZHANG You-lin(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi'an 710119,China;Shaanxi Forestry Technology Extension Station,Xi'an 710082,China;Shaanxi Shangluo Shengda Industrial Corporation Limited,Shangluo 726000,China;Shaanxi Chenggu County Agricultural Machinery Management Station,Hanzhong 723200,China)
出处
《天然产物研究与开发》
CAS
CSCD
2022年第5期739-749,共11页
Natural Product Research and Development
基金
陕西省农业领域重点产业链项目(2021ZDLNY04-05)
陕西省林业科学院科技创新计划(SXLK2021-0232)。
关键词
欧李
多糖
提取
结构表征
免疫调节活性
Cerasus humilis
polysaccharide
extraction
structure characterization
immunoregulatory activity
