摘要
为了研究纳豆芽胞杆菌高抑菌活性的最佳发酵条件,以苏云金芽孢杆菌和大肠杆菌为指示菌,采用滤纸片法,分别考察了培养时间、培养温度、培养基盐度、碳源、氮源及纳豆菌发酵粗提液浓度等因素对两种指示菌的抑制效果。结果表明:随着培养时间和粗提液浓度的提高,苏云金芽孢杆菌和大肠杆菌的抑菌圈直径先增后减;培养温度为37℃时苏云金芽孢杆菌的抑菌圈最大,42℃时大肠杆菌的抑菌圈最大;盐度为1%时,粗提液对苏云金芽孢杆菌和大肠杆菌的抑菌效果最好;碳源为牛肉膏时苏云金芽孢杆菌的抑菌圈最大,碳源为乳糖时大肠杆菌的抑菌圈最大;氮源为蛋白胨时苏云金芽孢杆菌的抑菌圈最大,氮源为硫酸铵时大肠杆菌的抑菌圈最大。正交法考察纳豆菌抑菌物质粗提液抑制两株指示菌的最优条件为培养时间36 h,粗提液浓度为100%,培养基盐度为1.5%。
In order to study the optimum fermentation conditions for the antibacterial activity of Bacillus natto,we took Bacillus thuringiensis and Escherichia coli as indicator bacterias and using filter paper method to study the inhibitory effect of antibacterial substance on two kinds of indicator bacteria about culture time,culture temperature,medium salinity,carbon source,nitrogen source and the concentration of the crude extract of natto antibacterial substance.The results showed that,With the increase of culture time and concentration of crude extract,the diameter of the inhibition zone of B.thuringiensis and E.coli increased firstly and then decreased.The incubation zone of B.thuringiensis was the largest at culture temperature of 37℃and the inhibition zone of E.coli was the largest at 42℃.When the salinity was 1%,the inhibition zone of B.thuringiensis and E.coli were the largest.When the carbon source was beef extract,the inhibition zone of B.thuringiensis was the largest and when the carbon source was lactose,the largest inhibition zone of E.coli was the largest.The inhibition zone of B.thuringiensis was the largest when the nitrogen source was peptone,and the inhibition zone of E.coli was the largest when the nitrogen source was ammonium sulfate.The optimum conditions for the inhibition of crude extract of Bacillus natto to two indicator strains by orthogonal test were 36 h culture time,100%concentration of crude extract and 1.5%salinity of medium.
作者
宋军霞
祁红兵
苏晓盈
SONG Jun-xia;QI Hong-bing;SU Xiao-ying(School of Geographic Sciences,Lingnan Normal University,Zhanjiang 524048,China;Life Sciences and Technology School,Lingnan Normal University,Zhanjiang 524048,China)
出处
《大豆科学》
CAS
CSCD
北大核心
2022年第2期203-208,共6页
Soybean Science
基金
国家星火计划(2015GA780051)
产业链协同创新类公共服务平台项目(湛海创2017CB82)
广东省岭南师范学院校内项目(2019)。
关键词
纳豆芽胞杆菌
抑菌活性
发酵条件
正交优化
Bacillus natto
antibacterial activity
fermentation conditions
orthogonal optimization
