摘要
背景:据报道,激素、酒精均可抑制骨髓间充质干细胞从而影响骨的形成与修复,而股骨头塌陷的发生与机械应力刺激、坏死组织修复能力相关,推测Piezo1可能是感应股骨头坏死塌陷的力学敏感蛋白。目的:观察力学感应蛋白Piezo1在激素性和酒精性股骨头坏死患者骨组织中的差异表达。方法:选取30例在2020年8月至2021年4月因股骨头坏死行人工全髋关节置换的患者,收集其股骨头标本及临床、影像资料。股骨头标本均为ARCOⅢ期;其中男20例,女10例;分为激素组15例,酒精组15例。苏木精-伊红染色观察股骨头标本骨组织形态结构变化;Westernblot检测骨组织中Piezo1蛋白的半定量表达;免疫组化检测Piezo1蛋白在骨组织中的定位表达。结果与结论:①苏木精-伊红染色结果发现激素及酒精组坏死区骨质结构紊乱、骨髓坏死,均有大量空虚的骨陷窝;②Western blot结果提示激素组患者股骨头骨组织的坏死区、硬化区和正常区Piezo1蛋白的表达均高于酒精组患者(P<0.05);③免疫组化结果提示激素性股骨头坏死组坏死区、硬化区和正常区阳性表达较酒精组更明显;④结果提示,Piezo1可能是感应股骨头坏死塌陷的力学敏感蛋白,并参与成骨的过程;激素性与酒精性股骨头坏死骨组织中Piezo1的差异表达可能与两者不同的骨形成修复特点有关。
BACKGROUND:Both hormones and alcohol can inhibit bone marrow mesenchymal stem cells and thus affect bone formation and repair.The occurrence of femoral head collapse is related to mechanical stress stimulation and the ability to repair necrotic tissue.Piezo1 may be a mechanosensitive protein that senses femoral head collapse due to osteonecrosis of the femoral head(ONFH).OBJECTIVE:To observe the differential expression of Piezo1 in osseous tissue of patients with steroid-and alcohol-induced ONFH.METHODS:Thirty patients who underwent total hip arthroplasty due to ONFH from August 2020 to April 2021 were enrolled,and their femoral head samples(a total of 30,all were ARCO III stage;20 males,10 females;15 cases in the steroid-induced ONFH group and 15 cases in the alcohol-induced ONFH group),clinical and imaging data were collected.The morphological and structural changes of bone specimens in necrotic area were observed by hematoxylin-eosin staining.The semi-quantitative expression of Piezo1 protein in osseous tissue was detected by western blot.The localization expression of Piezo1 protein in osseous tissue was detected by immunohistochemistry.RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that bone structure disorder,bone marrow necrosis and a large number of empty bone lacunae were found in the necrotic area of the steroid-and alcohol-induced ONFH groups.The results of western blot assay showed that the expression of Piezo1 in the necrotic,sclerotic and normal area of the femoral head in steroid-induced ONFH group was higher than that in the alcohol-induced ONFH group(P<0.05).The immunohistochemical results showed that the positive expression of Piezo1 in the necrotic,sclerotic and normal area in the steroid-induced ONFH group was more obvious than that in the alcohol-induced ONFH group.All these findings indicate that Piezo1 may be a mechanosensitive protein that senses ONFH collapse and may be involved in the process of osteogenic repair.The differential expression of Piezo1 in osseous tissue of patients with steroidand alcohol-induced ONFH may be related to their different repair characteristics of bone formation.
作者
魏腾飞
何晓铭
韦雨柔
詹芝玮
何敏聪
何伟
魏秋实
Wei Tengfei;He Xiaoming;Wei Yurou;Zhan Zhiwei;He Mincong;He Wei;Wei Qiushi(Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Guangdong Research Institute for Orthopedics&Traumatology of Chinese Medicine,Guangzhou 510378,Guangdong Province,China;Joint Center,the Third Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510378,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2023年第2期270-275,共6页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金面上项目(81873327),项目负责人:何伟
国家自然科学基金青年科学基金(82004392),项目负责人:何敏聪
2021年广东省教育厅普通高校重点领域专项(2021ZDZX2005),项目负责人:魏秋实
广州中医药大学“双一流”与高水平大学学科协同创新团队重大项目(2021XK05),项目负责人:何伟
广州中医药大学“双一流”与高水平大学学科协同创新团队培育项目(2021XK41),项目负责人:魏秋实
2021年广东省中医骨伤研究院开放课题重点项目(GYH202101-01),项目负责人:何伟
2021年广东省中医骨伤研究院开放课题重点项目(GYH202101-04),项目负责人:魏秋实。
关键词
股骨头坏死
激素
酒精
Piezo1
机械敏感性离子通道
塌陷
骨修复
成骨
破骨
osteonecrosis of the femoral head
steroid
alcohol
Piezo1
mechanosensitive ion channel
collapse
bone repair
osteogenesis
osteoclast
