摘要
目的:研究人参皂苷Rg2(G-Rg2)对离体大鼠胸主动脉平滑肌的作用,并且对其作用机制进行初步探讨。方法:采用离体血管环技术制备离体大鼠胸主动脉环模型,采用血管收缩率为考察指标,设置内皮完整组和内皮去除组,记录人参皂苷Rg2(G-Rg2)对去甲肾上腺素(NE)预收缩大鼠胸主动脉环张力的变化影响。分别以L-NAME、ODQ、Indomethacin预孵育内皮完整血管环后,考察人参皂苷Rg2(G-Rg2)对去甲肾上腺素(NE)预收缩血管环的舒张作用;加入10^(-3) mol/L的人参皂苷Rg2孵育去内皮血管后,用不同浓度的NE、KCl、CaCl_(2)收缩血管,观察血管的舒张作用。结果:与空白对照比较,10^(-5)、10^(-4)、10^(-3)、10^(-2) mol/L的人参皂苷Rg2(G-Rg2)能显著提高经NE预收缩的内皮完整的血管环的舒张率(P<0.01),且呈浓度依赖趋势;经LNAME、ODQ、Indomethacin预孵育内皮完整血管后,不同浓度的人参皂苷Rg2(G-Rg2)能显著增加LNAME、ODQ预孵育后内皮完整的血管环的舒张率(P<0.01);对Indom预孵育内皮完整血管舒张率无显著影响(P>0.05);经10^(-3)mol/L人参皂苷Rg2(G-Rg2)预孵育去内皮血管后,不同浓度的去甲肾上腺素(NE)与空白对照组相比,没有显著性差异(P>0.05)。经10^(-3) mol/L人参皂苷Rg2(G-Rg2)预孵育去内皮血管后,不同浓度的KCl、CaCl_(2)与空白对照组相比,均具有显著性差异(P<0.01)。结论:人参皂苷Rg2(G-Rg2)具有良好的舒张血管作用并且呈内皮依赖性,其机制可能与内皮依赖性环氧化酶途径和抑制电压依赖性钙离子通道的开放所导致的细胞外钙的内流有关。
Objective:To investigate the effect and mechanism of ginsenoside Rg2(G-RG2)on isolated rat thoracic aorta smooth muscle.Methods:Vitro rat thoracic aorta ring model was prepared by using vascular ring technique.The vascular shrinkage rate was used as the index.The effects of ginsenoside Rg2(G-RG2)on the tension of thoracic aorta ring in rats with endothelium intact and endothelium removed were recorded.The endothelial intact vascular rings were preincubated with Lname,ODQ and Indomethacin,respectively,and the diastolic effect of ginsenoside Rg2(G-RG2)on the vascular rings preconstricted by norepinephrine(NE)was investigated.The endothelial vessels were incubated with 10^(-3) mol/L ginsenoside Rg2,and the vessels were constricted with different concentrations of NE,KCl and CaCl_(2) to observe the vasodilation.Results:Compared with the control group,ginsenoside Rg2(G-RG2)at 10^(-5),10^(-4),10^(-3),10^(-2) mol/L significantly increased the diastolic rate of ne-preconstricted intact vascular rings(P<0.01)with a concentrationdependent trend.After the intact endothelial vessels were preincubated with Lname,ODQ and Indomethacin,different concentrations of ginsenoside Rg2(G-RG2)significantly increased the diastulation rate of intact endothelial vascular rings after L-NAME and ODQ pre-incubation(P<0.01).There was insignificant effect on endothelial intact vasodilation rate of Indom pre-incubation(P>0.05).After pre-incubation with 10^(-3)mol/L ginsenoside Rg2(G-RG2)to remove endothelial vessels,there was no significant difference between different concentrations of norepinephrine(NE)and the control group(P>0.05).After preincubation with 10^(-3) mol/L ginsenoside Rg2(G-RG2)to remove endothelial vessels,different concentrations of KCl and CaCl_(2) had significant differences compared with the blank control group(P<0.01).Conclusion:Ginsenoside Rg2(G-RG2)has a good vasodilatory effect in endotheliumdependent manner.Its mechanism might be related to the endothelium-dependent cycooxidase pathway and the inhibition of extracellular calcium influx caused by voltagedependent calcium channel opening.
作者
任煜
刘婷婷
祁冰洁
祝凌丽
REN Yu;LIU Ting-ting;QI Bing-jie;ZHU Ling-li(Research Center Laboratory,Anqing Medical College,Anqing 246052,China)
出处
《宜春学院学报》
2022年第3期20-25,82,共7页
Journal of Yichun University
基金
安庆医药高等专科学校自然科学研究项目(编号:2020ZR007)。
关键词
人参皂苷RG2
离体血管
钙通道
ginsenoside Rg2
in vitro blood vessel
calcium channel
