摘要
目的对淮南市发生的一起宋内志贺菌暴发疫情进行病原学溯源分析,并对菌株毒力基因和耐药谱进行检测。方法采集2020年8月淮南市一起感染性腹泻暴发疫情相关水样、粪便,进行多重病原体核酸检测和分离培养,对分离的宋内志贺菌进行毒力基因PCR检测、微量肉汤法药敏检测、脉冲场电泳和全基因组测序溯源分析。结果56份黏稀样粪便分离出38株宋内志贺菌(阳性率67.86%),血清型均为宋内志贺菌Ⅰ相;3份末梢水和井水革兰阴性菌(GN)增菌液荧光PCR检测志贺菌阳性;菌株毒力基因携带情况为侵袭性质粒抗原H(ipaH)阳性(38份),侵袭性质粒抗原H(ipaH)/侵袭相关基因(ial)阳性(31份),侵袭性质粒抗原H(ipaH)/侵袭相关基因(ial)/肠毒素2基因(sen)阳性(1份);耐药谱显示14种抗生素中9种100.00%耐药,仅亚胺培南、氯霉素、头孢他啶、环丙沙星为有效药物;36株菌XbaⅠ限制性酶切图谱带型完全一致,其中3株菌株基因组ST型分析均为ST152型;全基因组测序和分析验证了此次暴发由单一克隆群菌株引起,且揭示此次暴发分离株与数据库中3株中国菌株、1株韩国菌株聚集成一个大簇,而与其他国家的菌株距离较远。结论本起由水源污染单一宋内志贺菌克隆引起,分离株为低毒力株,呈多重耐药表型。
Objective To understand the virulence gene and drug resistance profile of Shigella sonnei outbreak in Huainan city,and conduct pathogenic traceability analysis.Methods Water samples and feces related to an infectious diarrhea outbreak in Huainan city in August 2020 were collected for multiple pathogen detection.Virulence gene,drug sensitivity,pulse-field gel electrophoresis and whole genome sequencing of Shigella isolates were analyzed respectively.Results 38 strains of Shigella sonnei were detected in 56 samples of mucilage feces with a positive rate 67.86%,and all serotypes were Shigella sonnei Phase I.Three strains of Shigella sonnei were detected by fluorescence PCR in the Gram-negative(GN)bacterial enrichment solution of terminal water and well water.Virulence genes were ipaH positive(38),ipaH/ial(31)and ipaH/ial/sen positive(1),respectively.The drug resistance spectrum showed that 9 of 14 antibiotics were 100%resistant,and only imipenem,chloramphenicol,ceftazidime and ciprofloxacin were effective drugs.XbaⅠrestriction enzyme map type of 36 isolates was completely consistent,and the ST type analysis of 3 strains was ST152.Whole genome sequencing and analysis verified that the outbreak was caused by a single clonal group of strains,and revealed that the isolates of the outbreak were clustered into a large cluster with 3 Chinese strains and 1 Korean strain in the database,far away from the strains of other countries.Conclusion The outbreak is caused by a single clone of Shigella sonnei,which are low virulence strains and have multiple drug resistance.
作者
刘江
孙永
张洁
曾凡荣
王小波
朱利杰
孙梦阳
余寿杰
Liu Jiang;Sun Yong;Zhang Jie;Zeng Fanrong;Wang Xiaobo;Zhu Lijie;Sun Mengyang;Yu Shoujie(Microbiological Laboratory,Huainan City Center for Disease Control and Prevention,Huainan 232033,China;Microbiological laboratory,Anhui Provincial Center for Disease Control and Prevention,Hefei 230601,China)
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2022年第4期437-442,共6页
Chinese Journal of Preventive Medicine
基金
“十三五”重大传染病防治项目 (2018ZX10713003-002-005)。
关键词
宋内志贺菌
全基因组测序
聚合酶链反应
药敏试验
Shigella sonnei
Whole genome sequencing
Polymerase chain reaction
Drug susceptibility
