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大麦GRX家族的全基因组鉴定与生物信息学分析

Genome-wide Identification and Bioinformatics Analysis of GRX Family in Barley
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摘要 谷氧还蛋白(glutaredoxin,GRX)是一类小分子氧化还原酶,这一家族的成员广泛存在于各种生物之中,并且参与了许多重要生化反应。根据活性位点的氨基酸序列,可以将这一家族分为CPYC型,CGFS型以及CC型三类,其中CC型GRX仅在高等植物中存在。本研究通过生物信息学方法,鉴定出了25个大麦GRX基因,其中4个属于CGFS型,3个属于CPYC型,18个属于CC型。除了染色体位置不明的一个基因之外,其余的24个基因分布在除了4号染色体之外的6个染色体上。大麦中的GRX家族成员分别在多个器官中高表达,主要集中体现在幼穗、花器官、生长中的分蘖、根以及衰老的叶片中。本研究对大麦中的GRX基因进行了鉴定,并结合水稻和拟南芥中的结果进行了对比和分析,为后续研究提供了一定的参考和依据。 Glutaredoxins(GRXs)are small oxidoreductases present in various organisms and participate in many important biochemical reactions.According to the active site,this gene family can be divided into CPYC type,CGFS type and CC type.The CC type GRXs only exist in higher plants.In this study,25 barley GRX genes were identified by bioinformatics methods,4 of them belong to CGFS type,3 belong to CPYC type,and 18 belong to CC type.Except for one gene whose chromosome location is unknown,the remaining 24 genes are distributed on 6chromosomes except chromosome 4.The Barley GRX genes are highly expressed in multiple organs,including developing inflorescences,floral organs,developing tillers,roots and senescing leaves.This study identified the GRX genes in barley,and compared with those in rice and Arabidopsis,providing a reference and basis for further research.
作者 张斯逸 余婧 梁婉琪 Zhang Siyi;Yu Jing;Liang Wanqi(School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai,200240)
出处 《分子植物育种》 CAS 北大核心 2022年第7期2126-2134,共9页 Molecular Plant Breeding
基金 国家自然科学基金项目(U19A2031)资助。
关键词 大麦 谷氧还蛋白 生物信息学 Barley Glutaredoxin Bioinformatics
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