摘要
目的 探讨可溶性PD-1(SPD-1)、细胞因子样蛋白1(CYTL1)在类风湿关节炎(RA)患者中的水平及其与免疫指标的相关性。方法 选取2019年10月至2020年10月该院收治的RA患者128例作为研究组,同期在该院体检的健康志愿者60例作为对照组。采用酶联免疫吸附试验(ELISA)分别检测两组SPD-1、CYTL1水平,采用多功能液相芯片分析平台检测血清肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)、白细胞介素-17(IL-17)、类风湿因子(RF)和C-反应蛋白(CRP)水平,采用流式细胞仪检测血清CD3+、CD4+、CD8+T细胞比例和B细胞比例。采用多元线性回归分析RA患者中SPD-1、CYTL1水平与部分免疫指标之间的相关性。结果 研究组SPD-1水平高于对照组(P<0.05),研究组CYTL1水平低于对照组(P<0.05);研究组TNF-α、RF、CRP水平及B细胞比例均高于对照组(P<0.05),研究组IFN-γ、IL-4、IL-17水平及CD3+、CD4+、CD8+T细胞比例均低于对照组(P<0.05);多元线性回归分析结果显示,IL-4水平对RA患者的SPD-1、CYTL1水平产生影响(P<0.05)。结论 RA患者的SPD-1、CYTL1水平高于健康人,同时RA患者机体的部分免疫指标发生了一系列的生理化学变化,SPD-1、CYTL1水平与免疫指标IL-4水平存在相关性。
Objective To investigate the levels of soluble PD-1(SPD-1)and cytokine like protein 1(CYTL1)in the patients with rheumatoid arthritis(RA)and their correlation with the immune index.Methods A total of 128 patients with RA in this hospital from October 2019 to October 2020 were selected as the study group,and 60 healthy volunteers undergoing physical examination in the same period were selected as the control group.The enzyme linked immunosorbent assay(ELISA)was used to detect the levels of SPD-1 and CYTL1 in the two groups,and the multi-functional liquid chip analysis platform was used to detect serum tumor necrosis factor-α(TNF-α),γ-interferon(IFN-γ),interleukin-4(IL-4),interleukin-17(IL-17),rheumatoid factor(RF)and C-reactive protein(CRP)levels.The levels of serum CD3+,CD4+,CD8+T cells proportion and the proportion of B lymphocytes were detected by flow cytometry.The multiple linear regression analysis was used to analyze the correlation between the SPD-1 and CYTL1 levels with the partial immune indexes in RA patients.Results The SPD-1 level in the study group was higher than that in the control group(P<0.05),and the CYTL1 level in the study group was lower than that in the control group(P<0.05).The levels of TNF-α,RF and CRP and the B cells proportion in the study group were higher than those in the control group(P<0.05),while the IFN-γ,IL-4 and IL-17 levels and the proportion of CD3+,CD4+and CD8+T cells in the study group were lower than those in the control group(P<0.05).The multiple linear regression analysis results showed that IL-4 level affected the SPD-1 and CYTL1 levels in the patients with RA(P<0.05).Conclusion The levels of SPD-1 and CYTL1 in the patients with RA are higher than those in healthy people.At the same time,the partial immune indexes of the patients with RA have a series of physiological and chemical changes.The correlation exists between the levels of SPD-1 and CYTL1 with the immune index IL-4 level.
作者
黄艳艳
詹锋
肖璐
詹宇威
莫碧瑶
韩叶光
HUANG Yanyan;ZHAN Feng;XIAO Lu;ZHAN Yuwei;MO Biyao;HAN Yeguang(Department of Rheumatism and Immunity,Hainan Provincial People's Hospital/Affiliated Hainan Hospital of Hainan Medical University,Haikou,Hainan 570311,China;Department of Clinical Laboratory,Hainan Provincial People's Hospital/Affiliated Hainan Hospital of Hainan Medical University,Haikou,Hainan 570311,China)
出处
《国际检验医学杂志》
CAS
2022年第11期1286-1289,1294,共5页
International Journal of Laboratory Medicine
基金
海南省医药卫生科研项目(20A200387)
海南省自然科学基金项目(819QN347)
海南省临床医学中心建设项目资助[琼卫医函(2021)75号]。
