摘要
目的:比较白喉乌头生品、炮制品生物碱成分的含量变化及其细胞毒性。方法:将白喉乌头生品及哈萨克法炮制品(以下简称"炮制品")采用高效液相色谱法(High Performance Liquid Chromatography,HPLC)分析比较,测定炮制前后各提取液生物碱成分的变化;用MTT比色法分析比较不同浓度白喉乌头生品及炮制品提取液的细胞毒性。结果:白喉乌头经炮制,乌头碱及次乌头碱峰面积显著减少,降低率分别为45.1%和74.5%;去乙酰高乌甲素峰面积明显升高,升高率为62.2%;高乌甲素、冉乌头碱及去乙酰冉乌头碱峰面积基本不变;同时,有新的色谱峰产生,说明经炮制有新成分产生;白喉乌头生品及炮制品提取液浓度分别为在5000、2500、1250、625、312.5μg/mL给药48h,与白喉乌头生品提取液相比,其炮制品提取液细胞抑制率明显降低(P<0.05),白喉乌头生品及炮制品的IC50分别是1826.70μg/mL、3192.48μg/mL。结论:白喉乌头经炮制,主要成分的色谱峰面积发生了变化,同时有新色谱峰产生,其毒性成分乌头碱、次乌头碱峰面积明显减少,说明乌头碱、次乌头碱含量减少,通过MTT比色法检测细胞毒性,证明其达到减毒目的。
Objective:To compare the content changes and cytotoxicity of alkaloids in raw and processed(Kazakh processing method) Aconitum leucostomum Worosch.Methods:The raw and processed A.leucostomum were analyzed and compared by high performance liquid chromatography,and the changes of alkaloids in each extract before and after processing were determined.MTT assay was used to compare the cytotoxicity of raw and processed A.leucostomum.Results:The peak area of aconitine and hypaconitine decreased by 45.1 % and 74.5 %,The peak area of N-deacetyllappaconitine was increased 62.2 %.The peak areas of lappaconitine,ranaconitine and N-deacetylranaconitine were almost unchanged.New chromatographic peaks were produced,indicating that new components were produced after processing.Concentrations were 5000,2500,1250,625,312.5 μg/m L for 48 h,Compared with the raw A.leucostomum,the cell inhibition rate of the processed A.leucostomum was significantly decreased(P<0.05),The IC50 of raw and processed products were 1826.70 μg/m L and 3192.48 μg/m L,respectively.Conclusion:After processing,the chromatographic peak area of the main components changed,and new chromatographic peaks were generated.The peak areas of aconitine and hypaconitine,the toxic components,decreased significantly,indicating that the contents of aconitine and hypaconitine decreased.The cytotoxicity was detected by MTT method,and it was proved to achieve the purpose of reducing toxicity.
作者
吐尔克扎提·帕利哈提
聂继红
张海英
王德玺
兰卫
Tuerkezhati·Palihat;NIE Ji-hong;ZHANG Hai-ying;WANG De-xi;LAN Weil(College ofTraditional Chinese Medicine,Xinjiang Medical University,Urumqi,Xinjiang,830000,China;Affiliated Hospital of traditional Chinese Medicine,Xinjiang Medical University,Urumqi,Xinjiang,830000,China;Xinjiang Key Laboratory of Processing and Research of Traditional Chinese Medicine,Urumqi,Xinjiang,830000,China)
出处
《现代生物医学进展》
CAS
2022年第9期1630-1635,共6页
Progress in Modern Biomedicine
基金
国家自然科学基金项目(81160498)。
关键词
白喉乌头
炮制品
高效液相色谱法
毒性
H9C2心肌细胞
Aconitum leucostomum Worosch
Processed products
High Performance Liquid Chromatography
Toxicity
H9c2 car-diomyocytes
