摘要
目的:检测多囊卵巢综合征(PCOS)患者外周血长链非编码NTF3-5(Lnc-NTF3-5)表达水平,分析与胰岛素抵抗(IR)关系。方法:2017年12月—2020年12月本院妇产科治疗的108例PCOS患者为PCOS组,年龄匹配且同期体检的健康女性108例为对照组,采集空腹静脉血,分别采用实时荧光定量和酶联免疫吸附实验检测各组Lnc-NTF3-5、CRP水平,依据稳态模型评估法(HOMA-IR)指数将PCOS组分为IR组和NIR组,比较各组Lnc-NTF3-5的表达水平和HOMA-IR差异,Pearson法分析PCOS患者Lnc-NTF3-5表达水平与HOMA-IR、CRP、性激素水平的相关性。结果:PCOS组Lnc-NTF3-5、CRP水平均高于对照组,IR组Lnc-NTF3-5及HOMA-IR水平均高于NIR组(均P<0.05);PCOS组Lnc-NTF3-5的表达与HOMA-IR、CRP、促黄体生成素、睾酮、雌二醇水平均呈正相关,与卵泡刺激素水平呈负相关(均P<0.05)。结论:PCOS患者外周血Lnc-NTF3-5的表达上调,可能通过影响PCOS患者体的炎症反应和性激素分泌,在IR中发挥重要作用,为PCOS中IR的潜在机制并确定新的治疗靶点提供新思路。
Objective:To detect the expression of circulating long non-coding NTF3-5(Lnc-NTF3-5)in peripheral blood of patients with polycystic ovary syndrome(PCOS),and to study the correlation between the Lnc-NTF3-5 level of the patients and their insulin resistance(IR).Methods:108 patients with PCOS were included in study group during December 2018 to December 2020,and 108 healthy women with the matching age who underwent physical examinations were included control group during the same period.Fasting venous blood of the patients in the two groups were collected.Real-time fluorescence quantification and enzyme-linked immunosorbent assay were used to detect the levels of Lnc-NTF3-5 and CRP of the patients in the two groups,respectively.The patients in the study group were also divided into group A(the patients with IR)and group B(the patients without IR)according to homeostatic model assessment(HOMA-IR)index of the patients.The Lnc-NTF3-5 expression level and the HOMA-IR value of the patients were compared among these groups.Pearson method was used to analyze the correlation between the Lnc-NTF3-5 level of the patients with PCOS and their CRP and sex hormone levels,and their HOMA-IR value.Results:The levels of Lnc-NTF3-5 and CRP of the patients in the study group were significantly higher than those of the patients in the control group,and the Lnc-NTF3-5 level and the HOMA-IR value of the patients in group A were significantly higher than those of the patients in group B(all P<0.05).The expression of Lnc-NTF3-5 of the patients in the study group was positively correlated with their levels of CRP,luteinizing hormone,testosterone,and estradiol,and their HOMA-IR value,but was negatively correlated with their follicle stimulating hormone level(all P<0.05).Conclusion:The expression of Lnc-NTF3-5 in peripheral blood of the patients with PCOS is up-regulated,which may play an important role in their IR by affecting the inflammatory response and the sex hormone endocrine of the patients.It provides the new ideas for studying the potential mechanism of IR and the identification of new therapeutic targets of the patients with PCOS.
作者
李永乐
张丽
申小静
杨光
王婷婷
LI Yongle;ZHANG Li;SHEN Xiaojing;YANG Guang;WANG Tingting(Henan Institute of Reproductive Health Science and Technology,National Health Commission Key Laboratory of Birth Defect Prevention,Zhengzhou,Henan province,450000;The Third People's Hospital of Henan Prov-ince)
出处
《中国计划生育学杂志》
2022年第6期1278-1281,共4页
Chinese Journal of Family Planning
基金
河南省生殖健康科学技术研究院2021年基本科研费项目(JBKY2021005)。
