摘要
目的探究外周血CD4^(+)CD25^(+)CD127调节性T细胞(Treg)表达与新生儿血液感染的关系。方法分析2018年1月至2021年1月期间于濮阳市清丰县中医院娩出的497例新生儿中89例疑似血液感染的新生儿临床资料,根据细菌血培养诊断结果分为感染组21例与未感染组68例。比较两组外周血CD3^(+)、CD4^(+)、CD4^(+)CD25^(+)CD127 Treg表达水平,采用Logistic多元回归分析模型分析新生儿血液感染影响因素,采用受试者工作特征曲线(ROC)评估上述指标对新生儿血液感染的诊断效能。结果感染组外周血CD3^(+)、CD4^(+)表达水平显著低于未感染组,CD4^(+)CD25^(+)CD127Treg表达水平显著高于未感染组,差异均有统计学意义(P<0.05)。Logistic回归分析显示,外周血CD3^(+)、CD4^(+)、CD4^(+)CD25^(+)CD127 Treg均为新生儿血液感染的独立危险因素(P<0.05)。ROC曲线分析显示,CD3^(+)、CD4^(+)、CD4^(+)CD25^(+)CD127 Treg诊断新生儿血液感染的曲线AUC分别为0.891、0.909、0.854,截断值分别为59.85%、38.57%、8.87%,诊断效能良好(P<0.05)。结论外周血CD3^(+)、CD4^(+)、CD4^(+)CD25^(+)CD127 Treg表达情况与新生儿血液感染具有一定相关性,且上述各指标在新生儿血液感染诊断中均具有良好效能,可为新生儿血液感染及后续治疗措施实施提供一定参考。
Objective To explore the relationship between the expression of CD4^(+)CD25^(+)CD127 regulatory T cells(Treg)in peripheral blood and neonatal blood infection.Methods The clinical data of 89 neonates with suspected blood infection among 497 neonates who were delivered in Qingfeng Hospital of Traditional Chinese Medicine,Puyang City were analyzed between January 2018 and January 2021,and the neonates were divided into 21 cases in the infected group and 68 cases in the noninfected group according to the diagnostic results of bacterial blood culture.The expression levels of CD3^(+),CD4^(+)and CD4^(+)CD25^(+)CD127 Treg in peripheral blood were compared between the two groups.Logistic multiple regression analysis model was used to analyze the influencing factors for neonatal blood infection.Receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficiency of the above indicators for neonatal blood infection.Results The expression levels of CD3^(+)and CD4^(+)in peripheral blood in the infected group were significantly lower than those in the non⁃infected group(P<0.05),and the expression level of CD4^(+)CD25^(+)CD127 Treg was significantly higher than that in the non⁃infected group(P<0.05).Logistic regression analysis showed that peripheral blood CD3^(+),CD4^(+)and CD4^(+)CD25^(+)CD127Treg were independent risk factors for neonatal blood infection(P<0.05).ROC curve analysis showed that the AUC values of CD3^(+),CD4^(+)and CD4^(+)CD25^(+)CD127 Treg in the diagnosis of neonatal blood infection were 0.891,0.909 and 0.854,and the cut⁃off values were 59.85%,38.57%and 8.87%respectively,and the diagnostic efficiency was good(P<0.05).Conclusion The expression of peripheral blood CD3^(+),CD4^(+)and CD4^(+)CD25^(+)CD127 Treg has a certain correlation with neonatal blood infection,and the above indicators have good efficiency in the diagnosis of neonatal blood infection and can provide some reference for neonatal blood infection and subsequent treatment measures.
作者
王丽娟
王丽锋
刘雪杰
姚瑞敏
WANG Lijuan;WANG Lifeng;LIU Xuejie;YAO Ruimin(Department of Pediatrics,Puyang Oilfield Disease Control and Prevention Center,Puyang,Henan,China,457001;Department of General Surgery,Qingfeng Hospital of Traditional Chinese Medicine,Puyang,Henan,China,457001;Department of Pediatrics,Puyang Oilfield General Hospital,Puyang,Henan,China,457001)
出处
《分子诊断与治疗杂志》
2022年第5期836-839,共4页
Journal of Molecular Diagnostics and Therapy
基金
河南省医学科技攻关计划项目(201904013)
