摘要
该研究旨在通过乙酸酐法研究合成乙酰化地黄多糖,优化其工艺参数并评估乙酰化多糖的抗氧化活性。以地黄多糖乙酰基的取代度(D;)为评判依据,考察投料比(乙酸酐-地黄多糖)、反应时间和温度等因素对反应结果的影响。结合单因素试验和响应面试验法优化了合成乙酰化地黄多糖的工艺参数。采用红外光谱(IR)和扫描电镜(SEM)初步分析了乙酰化地黄多糖的结构特征,并进一步考察了乙酰化地黄多糖的体外抗氧化性。结果显示,合成乙酰化地黄多糖的最佳条件是乙酸酐-地黄多糖2.70,反应时间3.0 h,温度48℃,在此工艺条件下,乙酰化地黄多糖的D;为0.327。IR和SEM的实验结果显示地黄多糖乙酰化成功。体外抗氧化性实验表明:相较于未经乙酰化修饰的地黄多糖,乙酰化地黄多糖的抗氧化能力有了一定程度的改善。该研究为进一步开发应用地黄多糖提供了参考依据。
This study aims to acetylate Rehmannia glutinosa polysaccharides by acetic anhydride method, optimize process parameters and evaluate their antioxidant activity. With the degree of substitution(D;) as a criterion, the effects of reaction time, acetic anhydride-to-polysaccharides ratio and temperature were investigated. Process parameters were optimized by single-factor experiment and response surface methodology. The infrared spectroscopy(IR) and scanning electron microscopy(SEM) proved the successful acetylation and were employed to preliminarily analyze the structural characteristics of acetylated derivatives. The results showed that the D;was 0.327 under the optimal technological conditions, including m(acetic anhydride):m(R. glutinosa polysaccharides)=2.70, reaction time 3.0 h and temperature 48 ℃. Further, the antioxidant properties of acetylated derivatives were investigated in vitro and acetylation was found effective to improve the antioxidant activity of R. glutinosa polysaccharides. This study provides a reference for the further development and application of R. glutinosa polysaccharides.
作者
李瑾
张婷婷
蒲定涛
史亚军
左振宇
刘冲英
陈艳
贾晓斌
赵鹏
封亮
LI Jin;ZHANG Ting-ting;PU Ding-tao;SHI Ya-jun;ZUO Zhen-yu;LIU Chong-ying;CHEN Yan;JIA Xiao-bin;ZHAO Peng;FENG Liang(College of Pharmacy,Shaanxi University of Chinese Medicine,Xianyang 712046,China;School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第6期1539-1545,共7页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1706903,2018YFC1706904)
国家自然科学基金项目(81403101)
陕西省自然科学基金项目(2019JM-502)
陕西省中药基础与新药研究重点实验室基金项目(2017KF05,19JS019,21JS008)
陕西中医药大学学科创新团队项目(2019-YL11)
陕西省科技厅重点研发项目社会发展项目(2017SF-335,2020SF-315)。
关键词
地黄
多糖
乙酰化
响应面法
抗氧化
Rehmannia glutinosa
polysaccharides
acetylation
response surface methodology
antioxidant
