摘要
目的:探讨韦氏三联九针对横向定量牵拉模型大鼠闪光视觉诱发电位(FVEP)N2-P2波的影响。方法:将36只Wistar大鼠随机分组,27只均右眼手术造模。其中18只采用横向定量牵拉法制作大鼠外伤性视神经病变(TON)模型后随机分为韦氏三联九针组和模型组各9只,另外9只为假手术组,仅暴露视神经,不做牵拉处理。另有9只为正常对照组,不予任何干预。造模次日,韦氏三联九针组行韦氏三联九针治疗,每次20 min,1次/d,共14 d,模型组、假手术组与正常对照组不干预。韦氏三联九针代表穴:一联:“睛明”、“承泣”;二联:“丝竹空”透“太阳”;三联:“风池”、“太冲”。于1、7、14 d时观察各组FVEP情况。结果:与模型组相比,1 d时韦氏三联九针组N2波潜伏期、P2波潜伏期均缩短(P<0.05);7 d时,韦氏三联九针组N2波潜时缩短(P<0.01),N2-P2波振幅较模型组提高(P<0.05);14 d时,韦氏三联九针组P2波潜时缩短(P<0.05),N2-P2波振幅较模型组提高(P<0.05),组内前后比较,假手术组1、7、14 d的N2波潜伏期、P2波潜时与N2-P2波振幅差异均无统计学意义(P>0.05)。模型组的N2波潜伏期与P2波潜伏期延迟的情况在1~14 d期间一直未得到改善(P>0.05),且振幅一直在降低,于14 d出现与1 d比较显著差异(P<0.05)。而韦氏三联九针组N2波潜伏期延迟在7 d~14 d内才出现明显差异(P<0.05);其P2波潜伏期虽然在1 d~7 d内明显延迟(P<0.05),但14 d其P2波潜伏期延迟有所恢复,与1、7 d比较,差异无统计学意义(P>0.05)。韦氏三联九针组N2-P2波振幅在1~14 d期间亦无明显降低(P>0.05)。结论:本实验横向定量牵拉法TON大鼠模型建立成功,韦氏三联九针治疗TON模型大鼠,可缩减其FVEP电生理信号P2波峰延迟,提高N2-P2振幅,对视神经损伤的恢复呈现积极作用,可能与韦氏三联九针改善视神经传导功能、保护尚未变性坏死的神经节细胞的作用有关。
Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the transverse quantitative traction model.Methods:Thirty-six Wistar rats were randomly grouped,nine were in normal control group without any treatment,and the remaining 27 were surgically modelled in the right eyes.Eighteen of these rats were randomly divided into a Wei’s triple nine needling therapy group and a model group of nine rats each after the TON model was made using the transverse quantitative retraction method.The other nine rats were sham-operated,and only the optic nerve was exposed without retraction.On the next day of modelling,the Wei’s triple nine needling therapy group was treated with Wei’s Triplex Nine Needles for 20 minutes/1 time/1 day for 14 days.The model group,sham-operated group and normal control group were not intervened.Wei’s triple nine acupoints were represented as follows:1st link:"Jingming"(BL1)and"Chengqi"(ST1);2nd link:"Sizhukong"(SJ23)penetrating"Taiyang"(EX-HN5);and the third:"Fengchi"(GB 20)and"Taichong"(LV 3).The FVEP of each group was observed at 1 d,7 d and 14 d.The FVEP of each group was observed at 1 d,7d and 14 d.Results:Compared with the model group,the N2 wave latency and P2 wave latency were shortened in the Wei’s triple nine needling therapy at 1 d(P<0.05,P<0.01);at 7d,their N2 wave latency was shortened(P<0.01)and the N2-P2 wave amplitude was increased compared with the model group(P<0.05);at 14 d,their P2 wave latency was shortened(P<0.05)and the N2-P2 wave amplitude was increased.There was no statistical difference in N2 wave latency,P2 wave latency and N2-P2wave amplitude in the sham-operated group at 1 d,7 d and 14 d(P>0.05).The delayed N2 and P2 wave latencies in the model group did not improve from 1 d to 14 d(P>0.05)and the amplitude decreased throughout,showing a significant difference at 14 d compared to 1 d(P<0.05).In contrast,the N2 wave latency in Wei’s triple nine needling therapy group was not significantly different until 7 d to 14 d(P<0.05);its P2 wave latency,although significantly delayed from 1 d to 7 d(P<0.05),recovered at 14d and reached a level that was not statistically different from 1 d and 7 d(P>0.05).There was also no significant decrease in N2-P2 wave amplitude between 1d and 14d in Wei’s triple nine needling therapy group(P>0.05).Conclusion:In this experiment,the TON rat model was successfully established by the transverse quantitative retraction method,and the treatment of TON rats with Wei’s triple nine needling therapy reduced the P2 wave delay of the FVEP electrophysiological signal and increased the N2-P2 amplitude,which had a certain positive effect on the repair of optic nerve injury,probably related to its effect of improving the conduction function of the optic nerve and protecting the retinal ganglion cells that had not been degenerated and necrosed.
作者
李诗梦
周剑
韦企平
王军
孙武
王妍
王杰
夏燕婷
LI Shi-meng;ZHOU Jian;WEI Qi-ping;WANG Jun;SUN Wu;WANG Yan;WANG Jie;XIA Yan-ting(Beijing University of Chinese Medicine,Beijing 100029,China;Department of Ophthalmology,Dongfang Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100078,China;Department of Acupuncture,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China)
出处
《海南医学院学报》
CAS
2022年第12期887-892,共6页
Journal of Hainan Medical University
基金
国家自然科学基金资助项目(81904258)。
关键词
韦氏三联九针
外伤性视神经病变
横向定量牵拉法
闪光视觉诱发电位
大鼠
Wei’s triple nine needling therapy
Traumatic optic neuropathy
Transverse quantitative traction method
Flash visual evoked potential
Rat
