摘要
利用G-四链体DNA(T30695)催化Zn^(2+)插入到中卟啉IX(MPIX)中,引起荧光偏移的特点,建立了检测Zn^(2+)的方法。在40μmol/L MPIX、0.6μmol/L Pb^(2+)、5μmol/L T30695和1%Triton的最优实验条件下,该方法在Zn^(2+)浓度为0.5~5μmol/L范围内呈现良好的线性关系,相关系数R^(2)=0.95,检出限为73.5 nmol/L。离子选择性实验表明该方法对Zn^(2+)具有较好的选择性,用于实际样品测定,回收率在94.7%~100.4%之间。
A new biosensor was developed to detect zinc ion based on the principle that G-quadruplex DNA(T30695)catalyzed the insertion of zinc ion into mesoporphyrin IX(MPIX)and caused a shift in fluorescence spectra.Under optimal experimental conditions(40 μmol/L MPIX,0.6 μmol/L Pb^(2+),5 μmol/L T30695 and 1% Triton),this method had a good linear relationship in the range of 0.5-5 μmol/L(R~2=0.95),with a detection limit of 73.5 nmol/L.The selectivity experiments showed that this method had a good selectivity for zinc ion.Satisfactory recovery rates(94.7%-100.4%) were obtained,indicating that this method could be used in practical applications.
作者
田馨裕
刘焕
杨兴林
杨华林
TIAN Xinyu;Liu Huan;YANG Xinglin;YANG Hualin(College of Life Science,Yangtze University,Jingzhou 434025,China;Jinan Moslem Middle School,Jinan 250001,China;State Environmental Protection Key Laboratory of Synergetic Control and Joint Remediation for Soil&Water Pollution,Chengdu University of Technology,Chengdu 610059,China)
出处
《分析试验室》
EI
CAS
CSCD
北大核心
2022年第6期639-642,共4页
Chinese Journal of Analysis Laboratory
基金
国家环境保护水土污染协同控制与联合修复重点实验室开放基金项目(GHBK-2020-001)资助。
