摘要
目的:探索皖南蝮蛇毒蛋白抑制人乳腺癌细胞MCF-7转移的机制。方法:光学比浊法测定血小板聚集率筛分皖南蝮蛇毒蛋白组分;用蛇毒蛋白干预乳腺癌细胞MCF-7与血小板细胞共培养体系,显微镜下观察细胞形态学变化;CCK-8法判断细胞增殖情况;ELISA法检测平足蛋白和P选择素分泌情况。结果:血小板与MCF-7细胞共培养体系在光学显微镜下观察到血小板聚集于MCF-7表面;1 mg/mL皖南蝮蛇毒蛋白组分Ⅱ具有明显的促进血小板聚集作用(P<0.01);共培养体系中随蛇毒蛋白浓度增大MCF-7的增殖率逐渐下降,但差异无统计学意义(P>0.05);酶联免疫检测结果显示,12.5、25μg/mL蛇毒蛋白组平足蛋白含量降低、P选择素分泌增多(P<0.05)。结论:皖南蝮蛇毒蛋白抑制人乳腺癌细胞MCF-7转移的机制与皖南蝮蛇毒蛋白组分Ⅱ结合血小板表面C型凝集素受体2的能力大于肿瘤细胞表面平足蛋白结合血小板表面C型凝集素受体2的能力有关。
Objective:To investigate the mechanism of Agkistrodon venom protein deprived from southern Anhui area in inhibiting the metastasis of human breast cancer cell MCF-7.Methods:Protein component of Agkistrodon acutus venom from southern Anhui area was initially screened by light transmission aggregometry measurement.Then the snake protein was used to treat breast cancer cell MCF-7 that was co-cultured with the platelet cells.The morphology change of the cells was microscopically observed,and CCK-8 method was used to determine the cell proliferation.ELISA was performed to detect the secretion of podoplanin and p-selectin.Results:Platelet aggregation on the surface of MCF-7 was observed under the optical microscope in the co-culture system of platelet with MCF-7 cells.The results revealed that 1mg/mL Agkistrodon venom protein componentⅡobviously promoted platelet aggregation(P<0.01),and proliferation rate of MCF-7 was decreased with added snake venom protein concentration in co-culture system,yet the difference was not statistically significant(P>0.05).ELISA findings showed that the content of podoplanin was decreased and p-selectin was increased in groups treated with 12.5μg/mL and 25μg/mL snake venom protein(P<0.05).Conclusion:The mechanism of Agkistrodon acutus venom protein from southern Anhui area in inhibiting the metastasis of human breast cancer cell MCF-7 appears associated with greater ability of the snake protein componentⅡin binding to C-type lectin receptor-2 on platelet surface than that of tumor cell surface podoplanin in binding to C-type lectin receptor-2 on the platelet surface.
作者
李昱彤
孙瑶
赵丽虹
刘啸宇
陈磊
LI Yutong;SUN Yao;ZHAO Lihong;LIU Xiaoyu;CHEN Lei(College of Pharmacy,Wannan Medical College,Wuhu 2410002,China)
出处
《皖南医学院学报》
CAS
2022年第3期217-220,共4页
Journal of Wannan Medical College
基金
国家级大学生创新创业训练计划项目(201910368044)
皖南医学院重点科研项目培育基金(WK2016Z02)。
关键词
血小板
平足蛋白
C型凝集素受体2
皖南蝮蛇毒
platelet
podoplanin
C-type lectin-like receptor-2
Agkistrodon acutus venom from southern Anhui
