摘要
目的探讨低密度脂蛋白相关蛋白5(LRP5)基因多态性与绝经后女性2型糖尿病(T2DM)患者并发骨质疏松症(OP)的关系。方法将2019年8月至2021年9月于该院就诊或体检的绝经后女性共210例纳入研究,其中体检健康女性50例(A组),女性单纯T2DM患者50例(B组)、女性单纯OP患者52例(C组)、女性T2DM并发OP患者58例(D组)。采用基因测序技术对各组LRP5基因Q89R、A1330V位点的多态性进行检测。对B、C、D组与A组的糖代谢、骨代谢指标,骨密度(BMD),以及LRP5基因Q89R、A1330V位点基因型分布情况进行比较。分析D组LRP5基因A1330V位点多态性与糖代谢及骨代谢指标的关系。结果C、D组股骨颈BMD、腰椎L2~4 BMD均低于A组(P<0.05);B、D组空腹胰岛素(FINS)、糖化血红蛋白(HbA1c)均高于A组(P<0.05)。B、C、D组LRP5基因Q89R位点RR、QR、QQ型的分布与A组比较,差异无统计学意义(P>0.05);而B、C、D组LRP5基因A1330V位点VV、AV、AA型分布与A组比较,差异有统计学意义(P<0.05)。D组LRP5基因A1330V位点VV、AV、AA型的患者股骨颈BMD、腰椎L2~4 BMD、HbA1c比较,差异均有统计学意义(P<0.05)。结论LRP5基因A1330V位点多态性可能是绝经后女性T2DM患者并发OP的影响因素。
Objective To investigate the association of the low-density lipoprotein receptor-related protein 5(LRP5)gene polymorphisms with osteoporosis(OP)in postmenopausal women with type 2 diabetes mellitus(T2DM).Methods A total of 210 postmenopausal women who were treated in the hospital or had physical examinations from August 2019 to September 2021 were enrolled in the study,including 50 healthy women who had physical examination(group A),50 women with simple T2DM(group B),52 women with simple OP(group C)and 58 female T2DM patients with OP(group D).The polymorphisms of LRP5 gene Q89R and A1330V loci were detected by using gene sequencing technology.The glucose and bone metabolism indicators and bone mineral density(BMD)and genotypes of LRP5 gene Q89R and A1330V loci in groups B,C,D and A were compared,and the polymorphism of LRP5 gene A1330V locus in group D was analyzed.Analyse the relationship between the polymorphism of LRP5 gene A1330V locus in group D and the indicators of glucose metabolism and bone metabolism.Results Femoral neck BMD and L2-4 vertebra BMD in groups C and D were lower than those in group A(P<0.05),fasting insulin(FINS)and glycosylated hemoglobin(HbA1c)in groups B and D were higher than those in group A(P<0.05).The distribution of RR,QR and QQ types at the Q89R loci of LRP5 gene in groups B,C and D was not significantly different from that in group A(P>0.05),while the distribution of VV,AV and AA types at the A1330V loci of LRP5 gene was significantly different from that in group A(P<0.05).There were significant differences in the BMD of the femoral neck and the L2-4 vertebra,and HbA1c among the patients with VV,AV,and AA types at the A1330V locus of the LRP5 gene in the group D(P<0.05).Conclusion A1330V locus polymorphism of LRP5 gene may be an influencing factor of OP in postmenopausal women with T2DM.
作者
徐伟
何艳
王莉华
罗涌
XU Wei;HE Yan;WANG Lihua;LUO Yong(Department of Endocrinology,Three Gorges Hospital Affiliated to Chongqing University,Chongqing 404000,China)
出处
《国际检验医学杂志》
CAS
2022年第13期1552-1555,1561,共5页
International Journal of Laboratory Medicine
基金
重庆市科技计划指导性项目(wzstc-201903009)。
关键词
低密度脂蛋白受体相关蛋白5
多态性
绝经
2型糖尿病
骨质疏松症
low-density lipoprotein receptor-related protein 5
polymorphism
menopause
type 2 diabetes mellitus
osteoporosis
