摘要
热激蛋白HSP90是植物中一类高度保守的分子伴侣,参与细胞内蛋白质稳态调控、底物蛋白的激活和热激因子的调控,在高温干旱等逆境胁迫中发挥重要作用。从12个茄科植物基因组中共鉴定出130个HSP90基因,包含马铃薯的11个HSP90基因,马铃薯HSP90基因与番茄属植物的亲缘关系最近。马铃薯HSP90蛋白质基序较为保守;马铃薯HSP90基因启动子分析发现其包含植物激素、高温胁迫、干旱胁迫响应等顺式作用元件。通过对高温、干旱胁迫条件下的马铃薯HSP90基因进行qRT-PCR分析,热激后大多数HSP90基因显著上调,4个HSP90基因表达量极高;干旱胁迫下多数HSP90基因也呈上调趋势,其中1个HSP90基因表达量极高。STRING数据库预测出HSP90蛋白的22个伙伴蛋白,其功能涉及逆境胁迫应答、生长发育、信号转导等生物学过程;蛋白质互作网络分析发现5个高表达量HSP90基因参与调控内质网未折叠蛋白反应(UPR,unfolded protein response)、细胞内蛋白质降解与活性氧(reactive oxygen species,ROS)生成,在抵抗极端逆境胁迫中发挥重要作用。
Heat shock proteins(HSP90s)are highly conserved plant proteins that partieipate in cellu-lar homeostasis,client protein aetivation-and regulation of HSF activity.Therefore,HSP90s.may play an important role under high temperature and drought stresses.In this.study,we identified a total of 130 HSP9O genes from 12 Solanaceae plant genomes,which include 11 HSP9O genes in potato.Potato HSP9O genes were more elosely related to Lyopersicon species.The HSP90 proteins shared highly conserved protein motifs in ptato,the promoter region of HSP90 genes contained cis-elements which were related to plant hormones under high temperature and drought stresses.The potato HSP90 genes for qRT-PCR analysis revealed that most of the HSP90 genes were uP-regulated under high tempera-ture stress,especially,four HSP90 genes.were highly uP-regulated.Most of the potato HSP90 genes were uP-regulated under drought stress,only one HSP90 gene was highly uP-regulated.Furthermore,22 HSP9O partner proteins predicted by STRING database participated in the biological progresses such as adverse response,signal transduetion.growth and development.Additionally,a protein protein interaction network demonstrated that five highly expressed HSP9O.genes were involved in regulating unfolded protein response(UPR)of the endoplasmie reticulum.intracellar protein degradation,and ROS produetion.Taken together,these HSP90 genes play an important role in stress resistance.under adverse environmental stress.
作者
杨芳
乔岩
王永强
李茜
李丹
王亚士
柴薇薇
YANG Fang;QIAO Yan;WANG Yongqiang;LI Qian;LI Dan;WANG Yashi;CHAI Weiwei(College of Agriculture and Forestry,Longdong University,Qingyang Gansu 745000 China;Collaborative Innovation Centerfor Longdong Dryl and Crop Germplasm Improvement&Industrialization,Qingyang Gansu745000 China;Gansu KeyLaboratory of Protection and Utilization for Biological Resources and Ecological Restoration,Qingyang Gansu745000 China;Agronomy College,Gansu Agricultural University,Lanzhou 730030 China;College of Life Science and Engineering,Northwest Minzu University,Lanzhou 730030 China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2022年第6期690-702,共13页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(31960441)
甘肃省自然科学基金(18JR3RM236)
中央高校基本科研业务费专项资金(31920210056)
陇东学院博士科研启动基金(XYBY1706)。
关键词
茄科植物
马铃薯
高温干旱
HSP90基因家族
伙伴蛋白
Solanaceae plants
Potato
High temperature and drought
HSP90 gene family
Partner protein
