摘要
目的通过观察变应性鼻炎(allergic rhinitis,AR)小鼠的行为学和组织病理改变,检测血清IL-14、Ⅰ型变态反应的特异性免疫球蛋白E(IgE)浓度以及鼻黏膜IL-14表达水平,探讨AR病程是否会影响IL-14的表达。方法BALB/c小鼠分组,建立AR组和对照模型组,观察行为学和鼻部组织病理学改变,ELISA检测IgE和IL-14浓度,PCR检测IL-14基因表达情况。结果AR小鼠的抓鼻(P<0.01)和喷嚏(P<0.001)症状较对照组严重,AR组的病理改变较对照组显著,两组小鼠体重对于实验影响无显著性差异,AR组的血清IgE和IL-14浓度高于对照组(P<0.05),AR组鼻黏膜IL-14基因表达高于对照组(P<0.05)。结论体内的表达水平可以一定程度上反映AR的严重重度。
Objective To investigate whether the course of AR(allergic rhinitis)affects the expression of IL-14 by observing the behavioral and histopathological changes of AR mice,and detecting the concentration of serum IL-14 and specific immunoglobulin E(IgE)in type I allergy and the expression level of IL-14 in nasal mucosa.Methods BALB/c mice were divided into the AR group and the control group.Behavioral and nasal histopathological changes were observed,IgE and IL-14 concentrations were detected by ELISA,and IL-14 gene expression was detected by PCR.Results The symptoms of scratching the nose(P<0.01)and sneezing(P<0.001)of mice in the AR group were more serious than those in the control group.The pathological changes in the AR group were more significant than those in the control group.There was no significant difference in the influence of body weight of mice on the experiment between two groups.Serum IgE and IL-14 concentrations in the AR group were higher than those in the control group(P<0.05).The expression of IL-14 gene in nasal mucosa of the AR group was higher than that of the control group(P<0.05).Conclusion The expression level in vivo can reflect the severity of AR to some extent.
作者
李荣荣
瞿申红
张少杰
翁敬锦
黄雪颖
钟自玲
郑少川
Li Rongrong;Qu Shenhong;Zhang Shaojie;Weng Jingjin;Huang Xueying;Zhong Ziling;Zheng Shaochuan(Graduate School,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Department of Otolaryngology Head and Neck Surgery,People’s Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,Guangxi,China)
出处
《右江民族医学院学报》
2022年第3期330-335,共6页
Journal of Youjiang Medical University for Nationalities
基金
国家自然科学基金项目(81960186)。
