摘要
酶联免疫吸附法(ELISA)是检测食物过敏原最常用、最成熟的免疫学分析方法之一。ELISA通常分为夹心ELISA、竞争/抑制ELISA、直接ELISA和间接ELISA等几类,常用于牛乳、鸡蛋、花生、小麦和大豆等食物中过敏原的检测。基于抗原/抗体的特异反应将待测物与酶连接,酶与底物反应导致颜色变化,结合荧光检测技术实现定量检测。综述ELISA的原理和应用研究,旨在为食品中过敏原的快速、痕量检测提供技术方向,以期促进食物过敏原检测方法研究,有利于保障过敏患者的食品安全。
Enzyme-linked immunosorbent assay(ELISA)is one of the most commonly used and most mature immunological analysis methods for the detection of food allergens.ELISA is usually used for the detection of allergens in foods such as milk,eggs,peanuts,wheat and soybeans,which includes sandwich ELISA,competition/inhibition ELISA,direct ELISA and indirect ELISA.Based on the specific reaction of antigen/antibody,the analyte is connected to the enzyme.And the reaction of the enzyme and the substrate causes the change of color,then quantitative detection is achieve by fluorescence detection technology.The research progress of the principle and application of ELISA is reviewed,and looking forward to providing technical direction for rapid and trace detection of allergens in food,promoting the research of food allergen detection methods,and making sure the food safety of allergic patients.
作者
彭斓兰
朱永智
李靖
桑丽雅
王元凤
魏新林
PENG Lanlan;ZHU Yongzhi;LI Jing;SANG Liya;WANG Yuanfeng;WEI Xinlin(School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240;School of Life Sciences,Shanghai Normal University,Shanghai 200240;Hangzhou Nankai Rixin Biotechnology Co.,Ltd.,Hangzhou 310051)
出处
《食品工业》
CAS
2022年第3期213-217,共5页
The Food Industry
基金
上海市科技兴农重点攻关项目(2018-02-08-00-08-F01545)
上海市科委生物医药与农业重点项目(17391901302)。
关键词
过敏原
食物
酶联免疫
进展
allergen
food
enzyme-linked immunoassay
progress
