摘要
为了对苦水玫瑰醇提物的化学成分进行快速分析鉴定,本研究采用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF-MS)技术,选用Waters ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)色谱柱,流动相为0.1%甲酸水溶液(A)-0.01%甲酸乙腈溶液(B),梯度洗脱。在电喷雾离子源(ESI)负离子条件下,采集MS^(E)数据。根据精确相对分子质量、质谱碎片信息,结合相关文献及对照品比对,共检测出63个主要成分,包括26个黄酮类、11个多酚类(4个酚酸类及7个鞣质类)、7个三萜类、4个苯丙素类、8个有机酸类、1个环烯醚萜类、1个挥发油类化合物和5个未鉴定成分,其中柳穿鱼叶苷、草木樨苷、积雪草酸等28个化合物首次在该属得到鉴定。通过UPLC-Q-TOF-MS方法进一步完善了苦水玫瑰的化学成分,为药效物质基础研究提供了参考依据。
In order to quickly analyze and identify the chemical components of the alcohol extract of Rosa sertata×Rosa rugosa,ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was used in this study,Waters ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8μm)was used with The mobile phase is 0.1%formic acid aqueous solution(A)and 0.01%formic acid acetonitrile solution(B)and gradient elution.The MS^(E) data were collected under the negative ion condition of electrospray ion source(ESI).Sixty-three main components were detected according to the exact relative molecular mass,mass spectrometry fragmentation information,and related literature and reference materials,including 26 flavonoids,11 polyphenols(4 phenolic acids and 7 tannins),7 triterpenoids,4 phenylpropanoids,8 organic acids,1 iridoid terpene,1 volatile oil and 5 unidentified components.Twenty-eight compounds such as pectolinarin,melilotoside and asiatic acid were identified in this genus for the first time.UPLC-Q-TOF-MS method was used to further improve the chemical constituents of Rosa sertata×Rosa rugosa,which provided a reference basis for the basic study of pharmacodynamic substances.
作者
杜少波
陈晓文
董志强
谢惠春
DU Shao-bo;CHEN Xiao-wen;DONG Zhi-qiang;XIE Hui-chun(Academy of Life Science,Qinghai Normal University;Qinghai Province Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau;Qinghai Province Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of Qinghai-Tibetan Plateau,Xining 810008,China)
出处
《天然产物研究与开发》
CAS
CSCD
2022年第7期1129-1142,共14页
Natural Product Research and Development
基金
青海省科技厅科技成果转化专项项目(2020-NK-125)。
