摘要
【目的】探讨菌草栽培灵芝的理论依据和实践模式。【方法】分别以木屑、五节芒、芒萁、巨菌草等为栽培原料熟料袋式栽培灵芝,研究灵芝菌丝满袋时间、原基形成时间、生物转化率及营养成分,比较不同菌草和木屑栽培灵芝生长情况。采用国标通用方法对灵芝子实体营养成分、氨基酸成分和重金属含量进行测定,比较不同栽培原料对灵芝子实体营养成分的影响。【结果】4种配方的菌丝满袋时间和原基形成时间无显著差异,配方C(五节芒48%、芒萁30%、麦麸20%、石膏2%)子实体产量(27.03g)和生物转化率(13.52%)均略高于对照组(A配方即木屑配方),但无显著性差异;配方B(五节芒78%、麦麸20%、石膏2%)和配方D(芒萁30%、巨菌草48%、麦麸20%、石膏2%)粗蛋白显著高于木屑配方(P<0.05),配方C粗蛋白含量略高于木屑配方,但无显著性差异;配方C多糖含量(12.01 mg·g^(-1))、三萜含量(1.01 mg·g^(-1))高于对照组木屑配方(10.2 mg·g^(-1)、0.80 mg·g^(-1));4种配方子实体中的氨基酸总量基本一致,且4种配方子实体重金属含量均符合国家标准以及绿色食品标准。【结论】菌草作为灵芝栽培基质与木屑作为灵芝栽培基质相比具有成本低、来源足等优越性,为菌草栽培灵芝提供了理论依据。
【Objective】Growth and chemical composition of fruiting bodies of Ganoderma lucidum cultivated with varied substrates were studied.【Method】Sawdust,Miscantuus floridulus,Dicranopteris dichotomy,and/or Gaint Juncao grass were used as the main ingredients in formulating substrates for cultivating G.lucidum in bags.The hyphae filling time,biotransformation rate,and contents of nutrients and heavy metals in fruiting bodies were compared between the experimental and the conventional sawdust cultivations.Nutrients and heavy metals in fruiting body were determined using the national standard methods.【Result】No significant difference in the time taken for hyphae to fill the bag or primordium formation due to substrate formulation differentiation was observed.The fruiting body yield of 27.03 g and biotransformation rate of13.52%under Formula C that consisted of 48%M.floridulus,30%of D.dichotomy,20%of wheat bran,and 2%of gypsum were slightly,but not significantly,higher than those of control.Under Formula B(78%M.floridulus,20%wheat bran,and 2%gypsum)or Formula D(30%D.dichotomy,48%giant Juncao,20%wheat bran,and 2%gypsum),the fruiting bodies harvested had significantly higher crude protein than control(P<0.05),but not under Formula C.The content of 12.01 mg·g^(-1) on polysaccharides and 1.01 mg·g^(-1) on triterpene in the fruiting bodies grown on Formula C(48%M.floridulus,30%D.dichotomy,20%wheat bran,and 2%gypsum)were significantly higher than those of control at 10.20 mg·g^(-1) and 0.80 mg·g^(-1),respectively.The differently formulated substrates resulted in basically same amounts of amino acids in the mushrooms but below the safety limits of heavy metals according to the National Food Safety Standards and Green Food Standards.【Conclusion】Juncao appeared to provide significant advantages as a substrate for G.lucidum cultivation over sawdust.
作者
刘艳玲
刘朋虎
李晶
王彤
齐彬
林冬梅
林占熺
LIU Yanling;LIU Penghu;LI Jing;WANG Tong;QI Bin;LIN Dongmei;LIN Zhanxi(National Engineering Research Center of JUNCAO Technology,Fuzhou,Fujian 350010,China;Life Science College,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350010,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2022年第5期609-616,共8页
Fujian Journal of Agricultural Sciences
基金
福建农林大学学科交叉融合发展先导专项(XKJC-712021030)
福建省科技计划重大专项(2021NZ029009)。
关键词
灵芝
菌草配方
子实体
营养成分
重金属
Ganoderma lucidum
Juncao formula
fruiting body
nutritional components
heavy metals
