摘要
目的 研究北柴胡Bupleurum chinense DC.地上部分苯丙素及其抗细胞增殖活性。方法 采用溶剂萃取、硅胶柱色谱、ODS柱色谱、Sephadex LH-20凝胶柱色谱、制备型HPLC对北柴胡地上部分醇提物进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用CCK-8法,研究各化合物抗MGC-803胃癌细胞增殖活性。结果 从中分离得到35种苯丙素,一些化合物对MGC-803细胞增殖具有抑制作用。结论 化合物8~9、11、13~16、18~19、21、23~24、32~35为首次从伞形科植物中分离得到,化合物4、7、10、12、20、27为首次从柴胡属植物中分离得到,化合物1、3、6、22、29、31为首次从该植物中分离得到。化合物2~3、6、9、11、16、19、21、23~24、26~30、32具有抗细胞增殖活性。
AIM To study the phenylpropanoids from the aerial part of Bupleurum Chinese DC.and their anti-cell proliferation activities.METHODS The extract from the aerial part of B.Chinese was isolated and purified by solvent extraction,silica gel column chromatography,ODS column chromatography,sephadex LH-20 gel column chromatography and preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The anti-MGC-803 gastric cancer cell proliferation activity of each compound was studied by CCK-8 method.RESULTS Thirty-five phenylpropanoids were isolated and identified.Some compounds had inhibitory effects on cell proliferation.CONCLUSION Compounds 8-9,11,13-16,18-19,21,23-24,32-35 were isolated from the family Umbelliferae for the first time,compounds 4,7,10,12,20,27 were isolated from genus Bupleurum for the first time,compounds 1,3,6,22,29,31 were isolated from this plant for the first time.Compounds 2-3,6,9,11,16,19,21,23-24,26-30,32 show anti-proliferation activity.
作者
刘艳
刘培
黄金
肖宗雨
潘娟
管伟
匡海学
杨炳友
LIU Yan;LIU Pei;HUANG Jin;XIAO Zong-yu;PAN Juan;GUAN Wei;KUANG Hai-xue;YANG Bing-you(Key Laboratory of Basic and Application Research of Beiyao,Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《中成药》
CAS
CSCD
北大核心
2022年第7期2176-2186,共11页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(81973440)
黑龙江省“头雁”团队(2019-2023)。
关键词
北柴胡
地上部分
苯丙素
分离鉴定
抗细胞增殖活性
MGC-803胃癌细胞
CCK-8法
Bupleurum chinense DC.
aerial parts
phenylpropanoids
isolation and identification
anti-cell proliferation activity
MGC-803 gastric cancer cells
CCK-8 method
