摘要
【目的】旨在建立‘红颜’草莓组织快繁体系。【方法】以‘红颜’草莓茎尖为试材,用茎尖组织培养法和RT-PCR方法,研究不同培养基对其茎尖萌发率、不定芽增殖、生根率等的影响,并检测常见的5种草莓病毒(草莓轻型黄边病毒、草莓斑驳病毒、草莓镶脉病毒、草莓皱缩病毒和黄瓜花叶病毒)。【结果】适宜‘红颜’草莓初代培养基为MS+6-BA 1 mg/L+NAA 0.2 mg/L+PVP 1 g/L,继代培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+PVP 1 g/L,生根培养基为1/2MS+IBA 0.4 mg/L,移栽基质为草炭、蛭石和珍珠岩(体积比为2∶1∶1)。利用RT-PCR方法检测草莓病毒的脱除效果,脱除率为64.1%。【结论】该试验获得稳定的‘红颜’草莓组织快繁体系,为草莓产业高质量发展提供基础。
【Objective】The system of tissue culture and rapid propagation of Fragaria ananssa cv.‘Benihoppe’was established.【Method】Taking the stem tip of Fragaria ananssa cv.‘Benihoppe’as the material,the effects of different media on the germination rate,proliferation and rooting rate of the stem tip were studied,and the five common strawberry viruses(SMYEV,SMoV,SVBV,SCV,CMV)were detected by RT-PCR method.【Result】The results showed that the suitable primary medium for Fragaria ananssa cv.‘Benihoppe’was MS+6-BA 1 mg/L+NAA 0.2 mg/L+PVP 1 g/L,and the subculture medium was MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+PVP 1 g/L,the rooting medium was 1/2MS+IBA 0.4 mg/L,and the transplanting medium was turf,vermiculite and perlite(Volume ratio is 2∶1∶1).The result showed that the elimination of strawberry viruses reached 64.1%.【Conclusion】The system of tissue culture and rapid propagation of Fragaria ananssa cv.‘Benihoppe’has been generated and laid foundation for high-quality development of the strawberry industry.
作者
刘羽丰
白莹雪
王秋实
陈卿然
闫哲
尚巧霞
LIU Yufeng;BAI Yingxue;WANG qiushi;CHEN Qingran;YAN Zhe;SHANG Qiaoxia(College of Bioscience and Resource Environment/Key Laboratory of Urban Agriculture(North China),Ministry of Agriculture and Rural Affairs,Beijing University of Agriculture,Beijing 102206,China;Beijing Vocational College of Agriculture,Beijing 100031,China)
出处
《北京农学院学报》
2022年第3期73-78,共6页
Journal of Beijing University of Agriculture
基金
北京市粮经作物产业创新团队项目(BAIC09-2021)。
关键词
‘红颜’草莓
茎尖培养
快繁
病毒检测
Fragaria ananssa cv.‘Benihoppe’
strawberry
stem tip culture
rapid propagation
virus detection
