摘要
目的 观察具核梭杆菌(Fn)感染与高龄食管鳞癌(ESCC)患者临床病理参数及预后的关系。方法 选择高龄(≥75岁)ESCC患者106例,分别应用Q-PCR及RNAscope法检测癌组织中Fn的感染情况,并分析两种方法的一致性。采用Q-PCR检测结果分析Fn感染与患者临床病理参数之间的关系。术后随访2年,记录患者的生存情况,比较Fn阳性与阴性患者的2年生存率和生存时间。采用COX回归法分析Fn感染对高龄ESCC患者预后的影响。结果 Q-PCR及RNAscope法分别检出Fn阳性65例(61.32%)、62例(58.49%),两种方法的检测结果具有显著一致性(Kappa值为0.941,P<0.05)。Fn阳性高龄ESCC患者男性、吸烟、饮酒、有ESCC家族史、有吞咽哽噎症状、分化程度为低分化、肿瘤侵及纤维膜、淋巴结转移阳性、临床分期为Ⅲ/Ⅳ期的比例均高于Fn阴性患者(P均<0.05)。Fn阳性高龄ESCC患者生存28例、死亡37例(2年生存率43.08%),阴性患者生存36例、死亡5例(2年生存率87.80%),Fn阳性高龄ESCC患者的2年生存率及生存时间均明显低于Fn阴性患者(P均<0.05)。Fn阳性是影响高龄ESCC患者预后的独立危险因素(P<0.05)。结论 有吸烟、饮酒史及ESCC家族史的男性高龄ESCC患者感染Fn的风险升高,且感染Fn的高龄ESCC患者预后更差。
Objective To observe the relationships between Fusobacterium nucleatum(Fn) infection and clinicopathological parameters and prognosis in elderly patients with esophageal squamous cell carcinoma(ESCC).Methods A total of 106 elderly ESCC patients(>75 years old) were selected,and Fn infection in cancer tissues was detected by QPCR and RNAscope,respectively,to analyze their consistency.The relationships between Fn infection and clinicopathological parameters were analyzed by Q-PCR.Patients were followed up for 2 years after surgery,and the survival of patients was recorded.The 2-year survival rate and survival time of patients with positive and negative Fn infection were compared.COX regression was used to analyze the effect of Fn infection on the prognosis of elderly ESCC patients.Results Sixty-five cases(61.32%) and 62 cases(58.49%) were positive for Fn infection by Q-PCR and RNAscope,respectively.The detection results of the two methods were significantly consistent(Kappa value was 0.941,P<0.05).The proportions of male,smoking,drinking,family history of ESCC,choking on swallowing,low differentiation,positive tumor invasion and fibromembrane,lymph node metastasis,and stage Ⅲ/Ⅳ clinical stage in elderly ESCC patients with positive Fn infection were higher than those with negative Fn infection(all P<0.05).In elderly ESCC patients with positive Fn infection,28 cases survived and 37 cases died(the 2-year survival rate was 43.08%);in elderly ESCC patients with negative Fn infection,36 cases survived and 5 cases died(the 2-year survival rate was 87.80%);the 2-year survival rate and survival time of elderly ESCC patients with positive Fn infection were significantly shorter than those of patients with negative Fn infection(all P<0.05).Positive Fn infection was an independent risk factor for the prognosis of elderly ESCC patients(P<0.05).Conclusion Elderly male ESCC patients with smoking,drinking and family history of ESCC have an increased risk of Fn infection,and elderly ESCC patients infected with Fn have a worse prognosis.
作者
李超伟
张哲源
何珂瑶
张家豪
刘怡文
周福有
LI Chaowei;ZHANG Zheyuan;HE Keyao;ZHANG Jiahao;LIU Yiwen;ZHOU Fuyou(Department of Thoracic Surgery,The Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003,China;不详)
出处
《山东医药》
CAS
2022年第23期30-35,共6页
Shandong Medical Journal
基金
河南省科技攻关项目(212102310670)
河南省医学科技攻关省部共建项目(LHGJ20200811,SBGJ202103099)
河南省安阳市重大科技专项(ZDKJJH2020006)。
关键词
食管鳞癌
具核梭杆菌
高龄
预后
临床病理参数
esophageal squamous cell carcinoma
Fusobacterium nucleatum
advanced age
prognosis
clinicopathological parameters
