摘要
江淮地区是我国重要的小麦产区之一。为明确该地区麦田日本看麦娘对精噁唑禾草灵的抗性情况,采用整株水平生物测定法检测了该地区的10个种群对精噁唑禾草灵的抗性水平;扩增和比对了高抗种群(AH-40、JS-19)、中抗种群(AH-41、JS-20)与敏感种群(AH-7)的ACCaseCT区域基因片段差异,并测定了GSTs、P450s酶抑制剂对精噁唑禾草灵的增效作用及4个抗性种群对8种除草剂的抗性。结果表明,高水平抗性种群数量占供试种群总数的50%,抗性指数为13.14~18.54;测序结果表明,高抗和中抗种群中均未发现已报道可引起靶标抗性的位点突变。AH-40种群在施用GSTs代谢酶抑制剂(灭草环、三聚氯氰、NBD-Cl)和P450s酶抑制剂(PBO、马拉硫磷)后均可显著提高其对精噁唑禾草灵的敏感性,增效倍数分别为1.68、1.68、2.45、1.64、2.03;JS-19种群在施用GSTs酶抑制剂(灭草环、三聚氯氰、NBD-Cl)后GR50有所下降,增效倍数为1.71、1.94、1.71;供试酶抑制剂未对其他抗性种群产生明显增效作用。抗性检测结果显示,4个抗性种群均对炔草酯和甲基二磺隆高抗,AH-41、JS-19高抗高效氟吡甲禾灵,JS-20高抗烯禾啶,但均对唑啉草酯、啶磺草胺、氟唑磺隆和异丙隆敏感。
Jianghuai region is one of the important wheat production areas in China.In order to clarify the occurrence of Alopecurus japonicas resistance,whole-plant bioassay was used to test the resistance level of 10 populations to fenoxaprop-P-ethyl in this region.The differences of ACCase CT gene fragments between highly resistant population(AH-40,JS-19),moderately resistant population(AH-41,JS-20)and sensitive population(AH-7)were amplified and compared,and the synergistic effects of GSTs and P450s enzyme inhibitors on speroxazolyl and the resistance of the four resistant populations to eight herbicides were determined.The results showed that the populations with high level resistance accounted for 50%of the total population,with resistance index ranging from 13.14 to 18.54;the sequencing results revealed that no mutations at the loci were reported to cause resistance to the target were found in any of the four populations.The AH-40 showed significant increase in susceptibility to fenoxaprop-P-ethyl after the application of metabolizing enzyme inhibitors of GSTs(tridiphane,cyanuric chloride,NBD-Cl)and P450s(PBO,malathion),with synergism ratio of 1.68,1.68,2.45,1.64 and 2.03.The GR50 of the JS-19 population was reduced by the application of GSTs enzyme inhibitors(tridiphane,cyanuric chloride,NBD-Cl)with multiplicative effects of 1.71,1.94,1.71,and no significant synergistic effect of the test enzyme inhibitors was made on the other resistant populations.All four tested resistant populations showed high levels of cross-resistance to clodinafop-propargyl and mesosulfuron-methyl,AH-41,JS-19 to high-efficiency haloxyfop-R-methyl,and JS-20 to sethoxydim.The tested populations were susceptible to pinoxaden,pyroxsulam,flucarbazone-sodium,and isoproterenol,isoproturon.
作者
许锦程
毕亚玲
李君君
戴魏真
许孟桃
谷承文
XU Jincheng;BI Yaling;LI Junjun;DAI Weizhen;XU Mengtao;GU Chengwen(College of Resource and Environment,Anhui Science and Technology University,Fengyang,Anhui 233100,China;College of Agriculture,Anhui Science and Technology University,Fengyang,Anhui 233100,China)
出处
《麦类作物学报》
CAS
CSCD
北大核心
2022年第7期892-901,共10页
Journal of Triticeae Crops
基金
2019年度高校优秀青年人才支持计划项目(gxyq2019060)
安徽省教育厅自然科学重点项目(KJ2017A511)
农业农村部2021年农产品质量安全监管专项(农药)(15217029)
安徽科技学院稳定人才项目(NXWD201604)。
关键词
江淮地区
小麦田
日本看麦娘
代谢酶抑制剂
增效作用
抗性
Jianghuai region
Wheat fields
Alopecurus japonicas
Enzyme inhibitors
Synergistic effect
Resistance
