摘要
目的探索介导肿瘤铁死亡过程中的关键基因GPX4在泛癌中的潜在价值。方法采用UALCAN和TIMER数据库分析GPX4基因的mRNA表达水平;cBioPortal分析GPX4在泛癌中的基因变异情况;GEPIA分析GPX4表达与患者生存之间的关系;LinkedOmics分析靶向调控GPX4基因表达的MIR种类及KEGG通路分析;TIMER和GEPIA数据库研究GPX4基因与免疫细胞浸润的相关性及与免疫细胞标记基因的相关性。结果GPX4基因在15种肿瘤组织中异常表达,12种癌组织中存在变异,其表达与结肠癌(COAD)、胃癌(STAD)及甲状腺癌(THCA)患者生存期相关,MIR-503及MIR-127共同正向调节COAD、STAD及THCA中GPX4的表达,14种MIR共同负向调节GPX4表达。在COAD、STAD及THCA中,GPX4参与氧化磷酸化、磷脂酰肌醇信号系统、赖氨酸退化、谷胱甘肽代谢、JAK-STAT信号通路及MicroRNAs调节等过程。在THCA中,GPX4与B细胞、CD4^(+)T、CD8^(+)T细胞及巨噬细胞浸润水平有相关性。结论GPX4基因的表达、突变及调节与COAD、STAD及THCA的发生发展、患者的预后、肿瘤免疫有一定的相关性,为进一步研究其在泛癌中的作用奠定了基础。
Objective GPX4 is identified as a key regulator of the ferroptosis in cancers.However its potential value still needs to be further explored.Methods The mRNA expression level of GPX4 was verified in UALCAN and TIMER databases.The GPX4 genetic alteration was analyzed with cBioportal.The relationship between GPX4 expression and patient survival was investigated via GEPIA.The types of Mir targeted the regulation of GPX4 gene expression and KEGG pathway were analyzed by LinkedOmics.TIMER and GEPIA were applied to investigate the relationship between GPX4 and immune cell infiltration and immune cell marker genes.Results The GPX4 gene is abnormally expressed in 15 kinds of tumor tissues.There are 12 kinds of mutations in tumors.GPX4 expression is associated with survival of colon cancer,gastric cancer and thyroid cancer.Mir-503 and Mir-127 jointly positively regulate the expression of GPX4 in COAD,STAD and THCA.14 kinds Mir jointly negatively regulated GPX4 expression.In COAD,STAD and THCA,GPX4 is involved in oxidative phosphorylation,phosphatidy linositol signaling system,lysine degradation,glutathione metabolism,JAK-STAT signaling pathway and Micro RNAs regulation.In THCA,GPX4 was correlated with B cells,CD4^(+)T cells,CD8^(+)T cells and macrophage infiltration.Conclusion The expression,mutation and regulation of GPX4 gene are correlated with the occurrence and development of COAD,STAD and THCA,the prognosis of patients and tumor immunity,which lays a good foundation for further in-depth study for GPX4 gene in Pan-Cancer.
作者
余水红
李帆
金耀
马军
王晓梅
龚莉
YU Shuihong;LI Fan;JIN Yao;MA Jun;WANG Xiaomei;GONG Li(Anqing Medical College,Anqing 246052,China;School of Medicine,Shenzhen University,Shenzhen 518061,China;College of Life Sciences,Anqing Normal University,Anqing 246052,China)
出处
《济宁医学院学报》
2022年第4期278-282,288,共6页
Journal of Jining Medical University
基金
安徽省高校优秀人才项目(gxyq2021266)
安徽省教育厅项目(2020jxtd158
2019cxtd012)。
