摘要
目的:确定蒙药肋柱花中獐牙菜苦苷的最优提取工艺条件。方法:以獐牙菜苦苷作为指标性成分,采用HPLC法和正交试验来验证獐牙菜苦苷的最佳提取工艺,选取乙醇浓度、料液比、提取时间、提取次数4个因素,设计L9(34)正交试验,确定肋柱花中獐牙菜苦苷的最佳提取工艺。结果:肋柱花中獐牙菜苦苷的最佳提取工艺为乙醇提取浓度60%,料液比1∶10,回流提取1次,每次提取2 h。结论:优化得到的肋柱花獐牙菜苦苷提取工艺参数合理可行,质量稳定可控。
Objective To determine the optimal extraction conditions of swertiamarin from Lomatogonium rotatum(L.)Fries ex Nym.Methods swertiamarin was chosen as the examined component,HPLC and orthogonal design were used to optimize the extraction process of Lomatogonium rotatum.The L9(34)orthogonal test was designed to determine the optimal extraction process of swertiamarin from Lomatogonium rotatum by four extraction conditions ethanol concentration,ethanol volume,extraction time and extraction frequency.Results The optimal extraction conditions of swertiamarin were as follows:ethanol concentration 60%,solid-liquid ratio 1∶10,reflux extraction once for 2 hours each time.Conclusion The optimized extraction parameters of swertiamarin from Lomatogonium rotatum were reasonable and feasible,the quality was stable and controllable.
作者
额尼荣贵
于红珍
巴根那
付明海
ENI Ronggui;YU Hongzhen;BA Genna;FU Minghai(School of Mongolian Medicine,Inner Mongolia University for Nationalities,Tongliao 028000,China;NMPA Key Laboratory for Quality Control of Traditional Chinese Medicine(Mongolian Medicine),Tongliao 028000,China)
出处
《中国民族民间医药》
2022年第15期30-34,共5页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
国家自然科学基金项目(81803845)
蒙药标准化研究国际合作科技创新项目(MDK2018056)
内蒙古自治区本科直属高校2019年“双一流”建设专项资金蒙药科研创新团队建设项目(190301)
内蒙古民族大学2019级硕士研究生科研创新项目(编号:NMDSS2110)。
关键词
肋柱花
獐牙菜苦苷
正交试验
提取工艺
Lomatogonium rotatum
Swertiamarin
Orthogonal Design
Extraction Process
