摘要
目的:研究Synaptotagmin 1基因敲除(Syt1^(+/-))对小鼠情绪行为的影响并初步探讨其可能机制。方法:选取8周龄雄性Syt1^(+/-)小鼠及同窝野生型(WT)小鼠各5只,采用免疫荧光染色方法观察小鼠前额叶皮层、海马、杏仁核、伏隔核、纹状体和腹侧被盖区等6个脑区中Syt1的表达;选用8周龄雄性Syt1^(+/-)小鼠9只,以及WT小鼠10只为对照,通过旷场实验、高架十字迷宫实验和强迫游泳实验检测比较成年Syt1^(+/-)小鼠和WT小鼠的焦虑样行为;另选用8周龄雄性Syt1^(+/-)小鼠及WT小鼠各5只,检测小鼠前额叶皮层、海马和杏仁核的谷氨酸含量。结果:与WT小鼠相比,Syt1^(+/-)小鼠在前额叶皮层、海马、杏仁核、伏隔核、纹状体和腹侧被盖区Syt1阳性细胞数目显著减少(P<0.01);Syt1^(+/-)小鼠在旷场中总移动距离显著减少(P<0.01),并更偏爱在外周区域活动(P<0.01),对中心区域的探索欲望显著下降(P<0.01);Syt1^(+/-)小鼠更偏好待在封闭安全环境中(P<0.01),开臂探索次数(P<0.05)和在其中运动的时间显著减少(P<0.01);Syt1^(+/-)小鼠在强迫游泳实验中不动时间明显增加(P<0.01);同时,Syt1^(+/-)小鼠杏仁核中谷氨酸的含量显著增加(P<0.01)。结论:Syt1基因敲除可以引起小鼠显著的焦虑样行为,推测与杏仁核中谷氨酸含量增加有关。
Objective:To study the effects of Synaptotagmin1 gene knockout(Syt1^(+/-))on emotional behavior in mice and explore its possible mechanisms.Methods:Five 8-week-old male Syt1^(+/-)mice and five wild-type(WT)mice in the same litter were selected.The expressions of Syt1 in 6 mice brain regions of prelimbic cortex(PL),hippocampus(HIP),amygdala(AMY),accumbens nucleus(ACB),caudoputamen(CP)and ventral tegmental area(VTA)were detected by Immunofluorescence staining.Nine 8-week-old male Syt1^(+/-) mice and ten WT mice were selected as controls.The anxiety-like behaviors of adult Syt1^(+/-) mice and WT mice were detected by open field test,elevated plus maze test and forced swim test.In addition,five 8-week-old male Syt1^(+/-) mice and five WT mice were selected to detect the glutamate content in prelimbic cortex,hippocampus and amygdala.Results:Compared with WT mice,the number of Syt1 positive cells in PL,HIP,AMY,ACB,CP and VTA were decreased significantly in Syt1^(+/-) mice(P<0.01);Syt1^(+/-) mice had less total movement distance in open field test(P<0.01),more preference for peripheral area(P<0.01)and less desire to explore the central platform(P<0.01),while Syt1^(+/-)mice preferred to stay in a closed and safe environment(P<0.01);the number(P<0.05)and the time spent in open-arm explorations(P<0.01)were reduced significantly;the immobile time of Syt1^(+/-) mice was increased in the forced swim test(P<0.01).Meanwhile,the concentration of glutamate in the amygdala of Syt1^(+/-) mice was increased significantly(P<0.01).Conclusion:Syt1 gene knockout leads to significant anxiety-like behavior in mice,which is deduced that related to the increase of glutamate content in the amygdala.
作者
武南南
封芮芮
张宇
李光
曹济民
殷丽天
WU Nan-nan;FENG Rui-rui;ZHANG Yu;LI Guang;CAO Ji-min;YIN Li-tian(Department of Physiology,Shanxi Medical University,Ministry of Education Key Laboratory of Cell Physiology,Shanxi Provincial Key Laboratory of Cell Physiology,Taiyuan 030001;Institute of Cardiovascular Medicine,Southwest Medical University,Ministry of Education Key Laboratory of Medical Electrophysiology,Luzhou 646000,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2022年第2期97-101,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金(81601167,81670313,82170523)
山西省回国留学人员科研资助项目(2020-080)
山西省基础研究计划面上项目(20210302123304)
山西省“1331工程”提质增效建设计划项目(1331KFC)
山西省优秀研究生创新项目(2021Y407)。
