摘要
文章以脂多糖(LPS)诱导的急性肺损伤小鼠为动物模型,在相同原料来源和剂量下,比较研究灵芝多糖(Ganoderma lucidum polysaccharides, GLP)和灵芝三萜(Ganoderma lucidum triterpenoids, GLT)对LPS致小鼠急性肺损伤的预防作用及分子机制。结果表明,GLP和GLT均可显著降低急性肺损伤小鼠肺脏器指数、湿/干质量比、病理学损伤、中性粒细胞浸润、巨噬细胞浸润和炎症因子表达水平,改善急性肺损伤。电泳迁移率变动分析(electrophoretic mobility shift assay, EMSA)显示,GLP和GLT均可显著降低急性肺损伤小鼠肺组织中核因子κB(nuclear factor-kappa B,NF-κB)和激活蛋白-1(activator protein-1,AP-1)的DNA结合活性,说明GLP和GLT对LPS致小鼠急性肺损伤的预防作用与抑制NF-κB和MAPKs信号通路的激活相关。分析GLP和GLT缓解小鼠急性肺损伤作用的各指标对比表明,GLP的护肺效果优于GLT(P<0.05或P<0.01)。
The preventive effect and molecular mechanisms of Ganoderma lucidum polysaccharides(GLP)and Ganoderma lucidum triterpenoids(GLT)on acute lung injury(ALI)in mice were investigated and compared under the same material dosage,where mice were administered with lipopolysaccharide(LPS)to establish the ALI model.The results showed that GLP and GLT could significantly alleviate the organ index,wet/dry weight ratio,pathological injury,neutrophil infiltration,macrophage infiltration and pro-inflammatory cytokines expression of lung tissue of ALI mice.Electrophoretic mobility shift assay(EMSA)showed that GLP and GLT could remarkably reduce the DNA binding activities of nuclear factor-kappa B(NF-κB)and activator protein-1(AP-1)in the same ALI mice,suggesting that GLP and GLT could prevent LPS-induced ALI in mice via suppressing NF-κB and MAPKs signaling pathways.Based on the comparison of different parameters of ALI between GLP-and GLT-treated mice,it was concluded that the preventive effect of GLP on LPS-induced ALI was better than that of GLT(P<0.05 or P<0.01).
作者
施青青
王超群
张玉英
李强明
杨思林
罗建平
SHI Qingqing;WANG Chaoqun;ZHANG Yuying;LI Qiangming;YANG Silin;LUO Jianping(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China;Anhui Huaxin Biopharmaceutical Co.,Ltd.,Jieshou 236500,China)
出处
《合肥工业大学学报（自然科学版）》
CAS
北大核心
2022年第8期1139-1146,共8页
Journal of Hefei University of Technology：Natural Science
基金
安徽省科技重大专项资助项目(18030801112
201903a07020020)。
关键词
灵芝
多糖
三萜
急性肺损伤
炎症
Ganoderma lucidum
polysaccharide
triterpenoid
acute lung injury(ALI)
inflammation
