摘要
以香菇基因组数据为基础,对其细胞核基因组的密码子偏好性及偏好性形成因素进行分析。香菇细胞核编码基因的GC含量为48.66%,同义密码子第3位中碱基T(T3s)的出现频率最高(36.07%),同时高表达基因的GC、GC3以及GC3s含量均显著高于基因组水平;相关性分析结果显示基因表达与序列C3s含量、GC3、GC3s、GC含量、C含量以及CAI呈显著正相关;香菇基因组编码序列中共29个高频密码子,结合转录组筛选高表达和低表达基因的基础上鉴定出16个最优密码子;偏好性形成影响因素分析表明,香菇基因组密码子偏好性形成受中性突变和选择压力影响。本研究可以为采后香菇关键基因功能解析,开展香菇采后品质劣变机制研究提供参考。
Based on the genome data of Lentinula edodes,the codon preference and preference forming factors of its nuclear genomic genes were analyzed.The GC content of L.edodes nuclear coding genes was 48.66%,and the frequency of base T in the third position of synonymous codon was the highest(T3s,36.07%),and the contents of GC,GC3 and GC3s of highly expressed genes were significantly higher than the genome level.Correlation analysis showed that gene expression was significantly positively correlated with sequence C3s content,GC3,GC3s,GC contents,C content and CAI.The results of relative synonymous codon usage analysis showed that there were 29 high-frequency codons in the coding sequence of L.edodes genome,and 16 optimal codons were identified on the basis of screening high expression and low expression genes by transcriptome.The analysis of influencing factors of preference formation showed that the formation of codon bias in L.edodes genome was affected by neutral mutation and selection pressure.This study can provide a reference for the functional analysis of key genes and the study on the mechanism of postharvest quality deterioration of L.edodes fruiting body.
作者
邓冰
贾鸥阳
杨雨娴
孟俊龙
常明昌
刘靖宇
DENG Bing;JIA Ou-yang;YANG Yu-xian;MENG Jun-long;CHANG Ming-chang;LIU Jing-yu(College of Food Science and Engineering,Shanxi Agricultural University,Jinzhong 030801,China;Collaborative Innovation Center of Quality and Efficiency of Loess Plateau Edible Fungi in Shanxi,Jinzhong 030801,China;Key Laboratory of Shanxi Province for Loess Plateau Edible Fungi,Jinzhong 030801,China)
出处
《保鲜与加工》
CAS
2022年第8期8-15,共8页
Storage and Process
基金
国家自然科学基金(32102053)
山西省应用基础研究计划面上青年基金(201901D211380)
山西农业大学博士启动基金(2020BQ13)
农业农村部农产品贮藏保鲜重点实验室开放基金(Kf2022005)。
