摘要
为明确在抗生素胁迫下小麦对不同形态无机氮的吸收特性,通过水培的方式,以硝态氮(NO~--N)、铵态氮(NH~+-N)分别作为单一氮源,设置7个浓度水平的NO~--N、NH~+-N和5个质量浓度水平的罗红霉素(Roxithromycin, ROX)组合,以不含ROX处理为对照(CK),探究在ROX胁迫下小麦根系对NO~--N和NH~+-N的吸收动力学特征的变化。结果表明:(1)在不同质量浓度ROX胁迫下,小麦对NO~--N、NH~+-N的最大吸收速率随氮浓度的增高而增加,吸收特征符合Michaelis-Menten动力学模型;与CK相比,不同质量浓度ROX胁迫对2种形态无机氮的最大吸收速率均表现为促进作用;(2)随着ROX胁迫质量浓度的增加,小麦对NO~--N的最大吸收速率(V)持续增加,K值持续降低,吸收能力(α)持续增加;对NH~+-N的V、K值均持续增加,α值先增后降;(3)在10.0 mg/L ROX胁迫下,小麦对NO~--N的吸收能力高于对NH~+-N的吸收能力,而在0.1 mg/L、0.5 mg/L和1.0 mg/L ROX胁迫下,小麦对NH~+-N的吸收能力超过对NO~--N的吸收能力。
To clarify the absorption characteristics of different forms of inorganic nitrogen under antibiotic stress, hydroponics was used to cultivate wheats. Nitrate nitrogen(NO-N) and ammonium nitrogen(NH-N) were used as single nitrogen sources, respectively. Seven concentration levels of NO-N, NH-N and five concentration levels of roxithromycin(ROX) were established, and the treatment without ROX was used as control(CK). The absorption kinetics characteristics of NO-N and NH-N in wheat roots were explored under ROX stress. The results revealed that the maximum absorption rate of NO-N and NH-N in wheat increased with the increase of nitrogen concentration under different mass concentrations of ROX, and the absorption characteristics conformed to Michaelis-Menten equation. Compared with CK, different concentrations of ROX had a promoting effect on the maximum absorption rate of the two forms of inorganic nitrogen. With the increase of ROX concentration, the maximum adsorption rate(V)and absorptive capacity(α)of wheat to NO-N increased continuously, and the Kvalue decreased continuously. In addition, the Vand Kvalues of wheat to NH-N increased continuously, and the α value increased firstly and then decreased. Under the stress of 10.0 mg/L ROX, the absorption capacity of wheat to NO-N was higher than that to NH-N. On the contrary, under the stress of 0.1 mg/L ROX, 0.5 mg/L ROX and 1.0 mg/L ROX, the absorption capacity of wheat to NH-N was higher than that to NO-N.
作者
龚镇洪
余彬彬
黄盼盼
汪晓丽
钱晓睛
GONG Zhen-hong;YU Bin-bin;HUANG Pan-pan;WANG Xiao-li;QIAN Xiao-qing(Key Laboratory of Arable Land Quality Monitoring and Evaluation,Ministry of Agriculture and Rural Affairs,Yangzhou University,Yangzhou 225127,China;Key Laboratory of Pollution Processes and Environmental Criteria,Nankai University,Tianjin 300071,China;Key Laboratory of Original Agro-Environmental Pollution Prevention and Control,Ministry of Agriculture and Rural Affairs/Tianjin Key Laboratory of Agro-environment and Safe-product,Tianjin 300071,China;Dongying City Ecological Environment Bureau,Shandong,Dongying 257100,China)
出处
《江苏农业学报》
CSCD
北大核心
2022年第4期882-888,共7页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31500425)
江苏省自然科学基金青年科技人才项目(BK20150452)
农业部产地环境污染防控重点实验室/天津市农业环境与农产品安全重点实验室开放基金课题(16nybcdhj-4)
环境污染过程与基准教育部重点实验室(南开大学)开放基金课题(KL-PPEC-2016-3)。
关键词
罗红霉素
小麦
硝态氮
铵态氮
吸收动力学
roxithromycin
wheat
nitrate nitrogen
ammonium nitrogen
absorption kinetics
