摘要
目的探讨丹参酮ⅡA(TanⅡA)抑制肺癌细胞增殖作用。方法体外培养人肺癌细胞株A549、SK-MES-1、H446和H460,采用不同浓度的TanⅡA处理,对照组不做任何处理;采用CCK-8法测定细胞增殖,流式细胞术测定细胞凋亡,蛋白质印迹法测定细胞凋亡相关蛋白表达水平;比较不同浓度TanⅡA处理组和对照组的细胞凋亡率。结果TanⅡA抑制肺癌细胞增殖具有时间依赖性和浓度依赖性,TanⅡA浓度越高(t=4.503、2.114、2.103、3.567,均P<0.05)、处理时间越长(t=5.189、3.079、3.023、3.845,均P<0.05),4种肺癌细胞存活率越低。TanⅡA处理48 h后,A549、SK-MES-1、H446和H460细胞IC_(50)分别为(1.18±0.12)、(0.78±0.08)、(1.55±0.16)和(1.27±0.14)mg/mL。采用2.5 mg/mLTanⅡA处理48 h后,细胞凋亡率分别为(34.97±3.78)%、(37.62±2.48)%、(18.27±2.98)%和(19.17±2.30)%,与对照组[(4.86±0.36)%、(3.21±0.48)%、(3.25±0.26)%和(2.66±0.19)%]相比显著增加(P<0.05);Akt1蛋白表达水平降低,Bax/Bcl-2蛋白表达比率升高,caspase-9和caspase-3蛋白表达水平升高。结论TanⅡA可能通过影响细胞凋亡Bax/Bcl-2/caspase-9/caspase-3途径抑制肺癌细胞增殖,呈时间依赖性和浓度依赖性。
Objective To investigate the inhibition of tanshinoneⅡA(TanⅡA)on the proliferation of lung cancer cells.Methods Human lung cancer cell lines A549,SK-MES-1,H446 and H460 were cultured in vitro and treated with TanⅡA at concentrations of 0.3,0.6,1.3,2.5,5.0,10.0 mg/mL,while untreated cells served as controls.Cell proliferation was measured by using CCK-8 assay,and apoptosis was measured using flow cytometry,while the expression of apoptosis-related proteins was determined using Western blotting.The apoptotic rate of lung cancer cells was compared between TanⅡA-treated cells and untreated cells.Results TanⅡA inhibited lung cancer cell proliferation in a time dependent and concentration-dependent manner,and the survival rates of A549,SK-MS-1,H446 and H460 cells reduced with the concentration of TanⅡA(t=4.503,2.114,2.103 and 3.567;all P<0.05)and the duration of TanⅡA treatment(t=5.189,3.079,3.023 and 3.845;all P<0.05).The 48 h half maximal inhibitory concentrations(IC_(50)values)of TanⅡA were(1.18±0.12),(0.78±0.08),(1.55±0.16)and(1.27±0.14)mg/mL against A549,SK-MES-1,H446 and H460 cells,respectively.Following 48 h treatment with TanⅡA at a concentration of 2.5 mg/mL,the apoptotic rates of A549,SK-MS-1,H446 and H460 cells were(34.97±3.78)%,(37.62±2.48)%,(18.27±2.98)%and(19.17±2.30)%,which were significantly significantly higher than those of untreated cells[(4.86±0.36)%,(3.21±0.48)%,(3.25±0.26)%and(2.66±0.19)%,all P<0.05].Reduced Akt1 protein expression,elevated Bax/Bcl-2 expression ratio,and elevated caspase 9 and caspase-3 protein expression were detected in lung cancer cells treated with 2.5 mg/mL TanⅡA for 48 h relative to untreated cells.Conclusion TanⅡA may inhibit lung cancer cell proliferation in a time-and concentration-dependent manner via the Bax/Bcl-2/caspase-9/caspase-3 pathway.
作者
王江峰
杨诗雨
李健强
陈晟
WANG Jiangfeng;YANG Shiyu;LI Jianqiang;CHEN Sheng(Department of Thoracic Surgery,Cancer Hospital Affiliated to University of Chinese Academy of Sciences,Hangzhou,Zhejiang 310022,China;Hangzhou Medical College,Hangzhou,Zhejiang 310059,China)
出处
《预防医学》
2022年第9期893-897,共5页
CHINA PREVENTIVE MEDICINE JOURNAL
基金
浙江省中医药科学研究基金项目(2021ZB032)。
关键词
丹参酮ⅡA
肺癌
细胞增殖
细胞凋亡
tanshinoneⅡA
lung cancer
cell proliferation
cell apoptosis
