摘要
目的探讨血清肿瘤标志物联合液基薄层细胞检测(TCT)在宫颈癌诊断中的应用。方法选择2020年1月1日—2021年12月31日南通市肿瘤医院收治的100例宫颈癌患者作为观察组,另外选择同期100名在该院进行体检的健康体检者作为对照组。采用化学发光法对所有受检者进行血清糖类抗原125(CA125)、癌胚抗原(CEA)、鳞状细胞癌抗原(SCC)检测,使用Prep 2000系统对宫颈细胞进行TCT检测,包括巴氏染色、制片和观察。以病理活检诊断结果为“金标准”,比较两种检测方法的阳性率;对受试者工作特征曲线(ROC曲线)进行绘制,计算ROC曲线下面积(AUC),评估上述指标与方法单独或联合应用对宫颈癌的诊断价值。结果观察组宫颈癌患者的CA125、CEA、SCC水平均明显高于对照组〔CA125(kU/L):46.28±2.92比2.32±0.24,CEA(μg/L):7.45±0.48比1.01±0.12,SCC(μg/L):4.92±0.38比0.32±0.04,均P<0.05〕。观察组CA125、CEA、SCC指标和TCT方法单独及联合应用的阳性率均明显高于对照组(CA125:67.00%比1.00%,CEA:68.00%比1.00%,SCC:63.00%比2.00%,TCT:74.00%比1.00%,联合检测:92.00%比2.00%,均P<0.05);且观察组CA125、CEA、SCC指标与TCT方法联合应用的阳性率明显高于各指标与方法单独应用。ROC曲线显示,CA125、CEA、SCC指标与TCT方法联合应用的AUC为0.950,95%可信区间(95%CI)为0.915~0.985,且敏感度、准确度、阴性预测值和阳性预测值均明显高于各指标及方法单独应用。结论肿瘤标志物联合TCT检测在宫颈癌诊断中具有较高的应用价值,可提高疾病检出率。
Objective To explore the application of serum tumor markers combined with thin-prep cytology test(TCT)detection in diagnosis of cervical cancer.Methods One hundred patients with cervical cancer who were treated in Nantong Tumor Hospital from January 1,2020 to December 31,2021 were selected and included into the observation group,and other 100 healthy subjects who had physical examination in the hospital during the same period were selected as the control group.The serum levels of carbohydrate antigen 125(CA125),carcinoembryonic antigen(CEA)and squamous cell carcinoma antigen(SCC)were detected by chemiluminescence method in all subjects.TCT detection was performed by Prep 2000 system,including Pap staining,sectioning and observation on cervical cells.The diagnostic results of pathological biopsy were used as"gold standard"and the positive rates of different diagnostic methods for the disease were compared.The receiver operator characteristic(ROC)curve was drawn,the area under ROC curve(AUC)was calculated,and the diagnostic values of the combined and single detection of the indicators above were evaluated.Results The levels of CA125,CEA and SCC in observation group were significantly higher than those in control group[CA125(kU/L):46.28±2.92 vs.2.32±0.24,CEA(μg/L):7.45±0.48 vs.1.01±0.12,SCC(μg/L):4.92±0.38 vs.0.32±0.04,all P<0.05].The positive rates of CA125,CEA,SCC and TCT method in observation group were significantly higher than those in control group(CA125:67.00%vs.1.00%,CEA:68.00%vs.1.00%,SCC:63.00%vs.2.00%,TCT:74.00%vs.1.00%,combined detection:92.00%vs.2.00%,all P<0.05).The positive rate of combined detection of CA125,CEA,SCC and TCT method in the observation group was significantly higher than those of individual detection of indicators above.The ROC curve showed that the AUC of combined detection of CA125,CEA,SCC and TCT method was 0.950,95% confidence interval(95%CI)was 0.915-0.985,and the sensitivity,accuracy,negative predictive value and positive predictive value were significantly higher than those of each index alone.Conclusions The serum tumor markers combined with TCT method have higher clinical value in diagnosis of cervical cancer.Compared with single detection,combined detection can significantly improve the accuracy of disease detection.
作者
岑春伟
曹晓莉
张金业
陈健
Cen Chunwei;Cao Xiaoli;Zhang Jinye;Chen Jian(Department of Clinical Laboratory,Nantong Tumor Hospital,Nantong 226000,Jiangsu,China)
出处
《实用检验医师杂志》
2022年第2期174-177,共4页
Chinese Journal of Clinical Pathologist
关键词
宫颈癌
液基薄层细胞检测
肿瘤标志物
联合诊断价值
Cervical cancer
Thin-prep cytologic test
Tumor markers
Combined diagnostic value
