摘要
目的采用近红外光谱技术结合偏最小二乘法建立黄精多指标成分快速定量分析模型。方法采用紫外-可见分光光度法测定138批黄精多糖、皂苷含量;HPLC法测定5-羟甲基糠醛含量;近红外光谱技术采集近红外光谱图;偏最小二乘法建立定量预测模型,采用校正模型的相关系数、校正均方根误差和预测均方根对定量预测模型进行评价。结果黄精多糖、皂苷、5-羟甲基糠醛近红外定量模型相关系数分别为0.9970、0.9843、0.9839;校正均方根误差分别为0.276、0.122、0.004;预测均方根分别为0.198、0.075、0.006。验证集样品的平均相对偏差均小于2%。结论建立的近红外定量模型预测性良好,可用于黄精饮片实时监控和质量控制。
AIM To establish a model for rapid evaluation of multiple bioactive constituents of Polygonati Rhizoma by near-infrared spectroscopy and partial least squares.METHODS The totally selected 138 Polygonati Rhizoma extractions had their contents of total polysaccharides and saponins determined by ultraviolet-visible spectrophotometry,their 5-hydroxymethylfurfural determined by HPLC,and their overall spectra collected by near-infrared spectroscopy.The quantitative calibration model for three compounds established by partial least squares was evaluated further by the correlation coefficient,root-mean-square error of calibration and the root mean square of prediction of prediction model.RESULTS The total polysaccharides,saponins and 5-hydroxymethylfurfural found their correlation coefficients of prediction model being 0.9970,0.9843,and 0.9839,respectively;their root-mean-square error of calibration being 0.276,0.122,and 0.004,respectively;their root mean squares of prediction being 0.198,0.075,and 0.006,respectively.CONCLUSION The established model of near-infrared spectroscopy is stable,accurate and reliable for the simultaneous quantitative analyses of total polysaccharides,saponins and 5-hydroxymethylfurfural,highlighting the rapid determination of multiple bioactive constituents of Polygonati Rhizoma.
作者
吕悦
吴杭莎
韦飞扬
葛卫红
杜伟锋
李昌煜
张叶峰
LV Yue;WU Hang-sha;WEI Fei-yang;GE Wei-hong;DU Wei-feng;LI Chang-yu;ZHANG Ye-feng(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;Research Center for TCM Processing Technology,Zhejiang Chinese Medical University,Hangzhou 311401,China;Chinese Herbal Pieces Co.,Ltd.,Zhejiang Chinese Medical University,Hangzhou 311401,China;Academy of Chinese Medical Sciences,Zhejiang Chinese Medical University,Hangzhou 310053,China;Ningbo Chinese Herbal Pieces Co.,Ltd,Ningbo 315000,China)
出处
《中成药》
CAS
CSCD
北大核心
2022年第9期2878-2884,共7页
Chinese Traditional Patent Medicine
基金
国家重点研发计划(2018YFC1707001)
国家中药标准化项目(ZYBZH-H-ZY-45)
国家重点研发计划(342211A00401)
中华中医药学会青年人才托举工程项目(QNRC2-C12)
宁波市“科技创新2025”重大专项(20201ZDYF020069)
宁波重点研发计划(592219A00102)。
关键词
黄精
多糖
皂苷
5-羟甲基糠醛
近红外光谱
检测模型
Polygonati Rhizoma
polysaccharide
saponins
5-hydroxymethylfurfural
near-infrared spectroscopy
quantitative model
