摘要
目的:采用响应曲面法优化小叶红叶藤挥发油包合工艺,并对其包合物进行表征。方法:通过包合方法的考察,选择小叶红叶藤挥发油包合物的最佳制备方法;以挥发油包合率和包合物收率为评价指标,采用响应曲面法考察β-环糊精(β-cyclodextrin,β-CD)和小叶红叶藤挥发油投料比、包合温度、包合时间对包合工艺的影响,优化其包合工艺;通过显微鉴别、薄层色谱、紫外分光光度法和红外光谱法对包合物进行表征。结果:最佳包合工艺为β-CD和挥发油投料比为11.88 g/mL,包合温度为52.8℃,包合时间为1.72 h,综合评分为96.53分,包合物的预测值和理论值偏差较小,挥发油和β-CD已形成包合物。结论:响应曲面法适用于优化小叶红叶藤挥发油包合工艺。
Objective:To optimize the inclusion process of volatile oil of Xiaoye Hongyeteng(Rourea microphylla) by response surface method and characterize its clathrate compound.Methods:Through investigating the inclusion method,the best preparation method of volatile oil clathrate of Xiaoye Hongyeteng was selected;by taking the inclusion rate of volatile oil and the yield of clathrate compound as the evaluation indexes,response surface method was adopted to survey the rate of charge between β-CD and Xiaoye Hongyeteng,the inclusion temperature and the influence of inclusion time on the inclusion process,therefore to optimize the inclusion process;microscopic identification,thin layer chromatography,ultraviolet spectrophotometry and infrared spectroscopy were used to characterize inclusion complex.Results:The best inclusion process is:the rate of charge between β-CD and Xiaoye Hongyeteng is 11.88 g/mL,the inclusion temperature 52.8 ℃,inclusion time 1.72 h,comprehensive scores 96.53 points,the deviation between the predicted and theoretical values of inclusion complex is small,and the inclusion complex has been formed by volatile oil and β-CD.Conclusion:Response surface method applies to the optimization of inclusion process of volatile oil of Xiaoye Hongyeteng.
作者
袁彩英
刘本涛
黄嘉咏
严振硕
谢长江
霍丽妮
陈睿
YUAN Caiying;LIU Bentao;HUANG Jiayong;YAN Zhenshuo;XIE Changjiang;HUO Lini;CHEN Rui(School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China;Faculty of Chinese Medicine Science Guangxi University of Chinese Medicine)
出处
《西部中医药》
2022年第9期55-61,共7页
Western Journal of Traditional Chinese Medicine
基金
广西自然科学基金面上项目(2017JJA10613)
广西中青年教师基础能力提升项目(KY2016YB830)
广西中医药大学校级青年基金项目(QN14017)
广西中医药大学校级一般硕士研究生创新项目(YCSY20190072)
大学生创新创业课题(201913643004)。
关键词
小叶红叶藤
包合物
响应曲面法
Xiaoye Hongyeteng
clathrate compound
response surface method
