摘要
本研究通过平板对峙试验,自稻茬分离获得1株对禾谷镰刀菌具有良好抑菌活性的细菌hzq1601。经16S rDNA和gyrB基因序列比对结果表明,菌株hzq1601为解淀粉芽胞杆菌Bacillus amyloliquefaciens。菌株hzq1601与多菌灵相容性分析结果显示其可在1000 mg/L的多菌灵药液中正常存活。田间试验结果显示,10~8 cfu/mL的hzq1601菌液分别与50%多菌灵可湿性粉剂80 g/667m^(2)和70 g/667m^(2)联合施用的防治效果为87.1%和81.0%,与单独施用50%多菌灵可湿性粉剂100 g/667m^(2)处理的防治效果无显著性差异,但显著高于单独施用50%多菌灵可湿性粉剂80 g/667m^(2)和70 g/667m^(2)处理的防治效果,说明上述hzq1601菌液与50%多菌灵可湿性粉剂的2种组合对赤霉病的防治具有减药增效作用。对菌株hzq1601基因组的分析表明其基因组中存在13种次生代谢产物合成基因簇,显示其具有多方面的生防潜能。
In this study,a bacterial strain hzq1601 antagonizing against Fusarium graminearum was isolated from rice stubble by plate antagonism test,and was identified as Bacillus amyloliquefaciens according to its 16S rRNA and gyr B gene sequences.Compatibility analysis between this strain and carbendazim fungicide showed that the strain hzq1601 survived normally in 1000 mg/L carbendazim solution.The results of field experiment showed that the control effect of 10~8 cfu/mL hzq1601 fermentation broth respectively combined with 50%carbendazim WP at the dose of 80 g/667 m^(2) and 70 g/667 m^(2) on fusarium head blight were 87.1%and 81.0%,that was comparable to that of 50%carbendazim WP at the dose of 100 g/667 m^(2) alone,and significantly higher than those of 50%carbendazim WP at the dose of 80 g/667 m^(2) or 70 g/667 m^(2) alone.The results showed that the combined use of fermentation broth and carbendazim could reduce the application amount of carbendazim,improve the control effect on fusarium head blight.Genomic analysis suggested that the strain hzq1601 harbored 13 secondary metabolite biosynthetic gene clusters,which implied that B.amyloliquefaciens hzq1601 exhibited a multifaceted biocontrol potential.
作者
张震
邱海萍
柴荣耀
郝中娜
王教瑜
王艳丽
孙国昌
ZHANG Zhen;QIU Haiping;CHAI Rongyao;HAO Zhongna;WANG Jiaoyu;WANG Yanli;SUN Guochang(Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricultural Sciences/State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products/National Experiment Station for Crop Disease and Pest Resistance Evaluation,Hangzhou 310021,China)
出处
《中国生物防治学报》
CSCD
北大核心
2022年第3期673-680,共8页
Chinese Journal of Biological Control
基金
浙江省基础公益研究计划(LGN19C140011)
国家重点研发计划(2018YFD0200507)。
关键词
解淀粉芽胞杆菌
小麦赤霉病
多菌灵协同应用
生防机制
Bacillus amyloliquefaciens
wheat fusarium head blight
combined application of carbendazim
biocontrol mechanism
