摘要
目的:观察紫檀芪对大鼠心肌缺血/再灌注损伤(MI/RI)的影响,探讨其保护机制是否与调控心肌自噬有关。方法:72只SD大鼠随机分为六组(n=12),分别为假手术组、MI/RI组、阿司匹林组、紫檀芪低剂量组、紫檀芪中剂量组和紫檀芪高剂量组,干预14 d后,构建MI/RI模型,观察心肌组织病理改变、测定心肌梗死面积及心肌组织中细胞自噬信号相关蛋白Atg5、Beclin1、LC3Ⅰ/Ⅱ和p62蛋白表达水平,测定血清心肌损伤指标乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB),氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的含量。结果:心肌组织病理学检查提示,与假手术组比较,MI/RI组心肌纤维明显断裂,部分坏死,部分细胞结构模糊;与MI/RI组比较,各治疗组心肌纤维断裂明显减少,细胞结构较清晰(P<0.05)。与假手术组比较,MI/RI组心肌梗死面积、LDH、CK-MB及MDA显著升高(P<0.05),SOD、CAT及GSH-Px显著降低(P<0.05),Atg5、Beclin1及LC3Ⅰ/Ⅱ蛋白明显升高(P<0.05),p62蛋白显著降低(P<0.05);与模型组比较,各治疗组心肌梗死面积、LDH、CK-MB和MDA显著降低(P<0.05),SOD、CAT、GSH-Px明显升高(P<0.05),Atg5、Beclin1及LC3Ⅰ/Ⅱ蛋白表达降低(P<0.05),p62蛋白表达升高(P<0.05)。结论:紫檀芪预处理能减轻大鼠MI/RI,其机制可能与调控心肌细胞自噬、提高抗氧化能力,从而减轻心肌细胞损伤有关。
Objective:To observe the effects of Pterostilbene on myocardial ischemia/reperfusion injury(MI/RI)in rats,and explore whether its protective mechanism is related to the regulation of myocardial autophagy.Methods:72 SD rats were randomly divided into 6 groups(n=12),including sham operation group,model group,aspirin group,Pterostilbene low dose group,Pterostilbene medium dose group and Pterostilbene high dose group.MI/RI model was established 14 days after administration.The pathological changes of myocardial tissue were observed,the myocardial infarction size was determined,and the expression levels of autophagy signal-related proteins Atg5,Beclin1,LC3Ⅰ/Ⅱand p62 in myocardial tissue were determined.The contents of lactate dehydrogenase(LDH),creatine kinase isoenzyme(CK-MB),malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)in blood were determined.Results:Myocardial histopathological examination indicated that myocardial fiber fracture and inflammatory response occurred in MI/RI group compared with sham group.Compared with model group,all treatment groups showed improvement.Compared with sham group,model group had increased myocardial infarction area,LDH and CK-MB,increased MDA,decreased SOD,CAT and GSH-Px,increased Atg5,Beclin1 and LC3Ⅰ/Ⅱprotein,and decreased p62(P<0.05).Compared with model group,myocardial infarction size,LDH and CK-MB decreased,MDA decreased,SOD,CAT and GSH-Px increased,protein expressions of Atg5,Beclin1 and LC3Ⅰ/Ⅱdecreased,and p62 increased in all treatment groups(P<0.05).Conclusion:The pre-treatment of Pterostilbene can reduce MI/RI in rats,and the possible mechanism may be related to the regulation of autophagy of cardiomyocytes and the improvement of antioxidant capacity,thus alleviating the injury of cardiomyocytes.
作者
付华君
宋文英
田俊斌
李扬
FU Huajun;SONG Wenying;TIAN Junbin;LI Yang(Department of Anesthesiology,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)
出处
《陕西中医》
CAS
2022年第10期1342-1346,共5页
Shaanxi Journal of Traditional Chinese Medicine
基金
陕西省自然科学基础研究计划项目(2018JM7110)。
