摘要
目的探讨齐墩果酸(OA)对牙髓炎大鼠TLR9/MyD88/NF-κB p65信号通路的影响。方法建立牙髓炎大鼠模型,随机分为模型组、OA低剂量(40 mg/kg)组、OA中剂量(80 mg/kg)组、OA高剂量(160 mg/kg)组、甲硝唑(MTZ)(40 mg/kg)组,每组12只,另取12只大鼠设为对照组。分组给药后,检测大鼠根髓坏死比率;以HE染色检测大鼠牙髓组织病理情况;以试剂盒检测大鼠牙髓组织氧化应激因子SOD、MDA及血清促炎因子IFN-γ、IL-6水平;以免疫印迹实验检测大鼠牙髓组织TLR9/MyD88/NF-κB p65通路相关蛋白表达情况。结果与对照组比较,模型组大鼠牙髓组织发生严重病理损伤,根髓坏死比率、MDA、IFN-γ及IL-6水平、TLR9、MyD88、核内NF-κB p65蛋白表达显著升高(P<0.05),SOD水平显著降低(P<0.05)。与模型组比较,OA低、中、高剂量组及MTZ组大鼠牙髓组织病理损伤减轻,根髓坏死比率、MDA、IFN-γ及IL-6水平、TLR9、MyD88、核内NF-κB p65蛋白表达降低(P<0.05),SOD水平升高(P<0.05),且OA各组呈剂量依赖性(P<0.05),OA高剂量组与MTZ组比较,无显著差异(P>0.05)。结论OA可下调TLR9/MyD88/NF-κB p65通路蛋白,抑制牙髓炎大鼠氧化应激及炎症反应,减轻牙髓组织损伤,改善大鼠临床症状。
To investigate the effect of oleanolic acid(OA)on TLR9/MyD88/NF-κB p65 signaling pathway in rats with pulpitis,a rat model of pulpitis was established and randomly divided into model group,low-dose OA(40 mg/kg)group,middle-dose OA(80 mg/kg)group,high-dose OA(160 mg/kg)group,MTZ(40 mg/kg)group,with12 rats in each group,another 12 rats were recruited as control group.After group administration,the ratio of rat root pulp necrosis was calculated;the pathological condition of rat dental pulp tissue was detected with HE staining;the levels of oxidative stress factors SOD and MDA in rat dental pulp tissue and the levels of proinflammatory factors IFN-γand IL-6 in serum were detected with corresponding kits;the expression of TLR9/MyD88/NF-κB p65pathway related proteins in rat dental pulp tissue was detected with Western blotting.Data showed that compared with the control group,severe pathological injury occurred in dental pulp tissue of the model group,the ratio of root pulp necrosis,the levels of MDA,IFN-γand IL-6,and the expression of TLR9,MyD88 and nuclear NF-κB p65 proteins were significantly increased in the model group(P<0.05),while the SOD level was significantly reduced(P<0.05).Compared with the model group,thepathological damage of the dental pulp tissue of rats inthe low-,medium-and high-dose OA group and theMTZ group were reduced,the ratio of root pulp necrosis,the levels of MDA,IFN-γand IL-6,as well as the expression of TLR9,MyD88 and nuclear NF-κB p65 proteinswere significantly decreased(P<0.05),while the SOD level was increased(P<0.05).All these changes mentionedabove in the OA groups were dose-dependent(P<0.05),and there is no significant difference between high-dose OAgroup and MTZ group(P>0.05).In conclusion,OA can down-regulate the TLR9/MyD88/NF-κB p65 pathwayproteins,inhibit the oxidative stress and inflammation in rats with pulpitis,reduce pulp tissue damage,and thusmitigate the clinical symptoms of rats.
作者
贺俊成
江峥
陈林
HE Juncheng;JIANG Zheng;CHEN Lin(Department of Stomatology,Guangdong Maternal and Child Health Hospital,Guangzhou 510000,China;De-partment of Dental Pulp,Stomatological Hospital of Xiamen Medical College,Xiamen 361000,China;Department of Dentistry and Endodontics,Stomatological Hospital of Southern Medical University,Guangzhou 510000,China)
出处
《免疫学杂志》
CAS
CSCD
北大核心
2022年第10期869-875,共7页
Immunological Journal
基金
南方医科大学口腔医院科研培育计划项目(PY2020027)
广东省医学科学技术研究基金项目(B20210580)。
