摘要
目的研究贵州鼠尾草Salvia cavaleriei中抗菌活性成分。方法采用正相硅胶柱色谱、RP-18反相硅胶柱色谱、Sephadex LH-20凝胶柱色谱以及半制备高效液相色谱等多种手段进行分离纯化,并借助化合物的理化性质、质谱、核磁数据以及碳谱计算等方法确定化合物结构。采用微量肉汤稀释法测定化合物的最小抑菌浓度(minimum inhibitory concentration,MIC)。结果从贵州鼠尾草中分离得到11个化合物,分别鉴定为1,7-bis-(4′-hydroxyl-3′-methoxyphenyl)-5(R)-methoxyhepthan3-one(1)、姜烯酮A(2)、原儿茶醛(3)、对羟基苯甲醛(4)、oresbiusin B(5)、迷迭香酸(6)、bis(2-ethyloctyl)phthalate(7)、cylindol B(8)、2-(3',4'-二羟苯基)-1,3-胡椒环-5-醛(9)、苯甲醇葡萄糖苷(10)和1H-indole-3-carboxaldehyde(11),其中,化合物1为1个新的姜黄素类化合物。抗菌活性结果显示化合物1对青枯假单胞菌、产气荚膜梭菌有较好抑制作用,MIC为71.2μg/mL,而化合物2对青枯假单胞菌、产气荚膜梭菌、枯草芽孢杆菌的MIC为77.6μg/mL。结论化合物1为新化合物,命名为鼠尾草姜黄素。除化合物3和6外,其余化合物均为首次从该植物中分离得到,此外,化合物2、7、8和9为首次从唇形科植物中分离得到,化合物5为首次从鼠尾草属植物中分离得到。化合物1和2表现出明显的抗菌活性。
Objective To study the chemical constituents with antibacterial activity from Salvia cavaleriei(Labiatae).Methods The compounds were separated by silica gel column chromatography,ODS columns chromatography,and semi-preparative HPLC.The structures were identified by MS,NMR spectra and quantum chemical calculations.The minimum inhibitory concentration(MIC)of isolated compounds was determined by broth dilution method.Results Eleven compounds were isolated from S.cavaleriei,which were identified as 1,7-bis-(4′-hydroxyl-3′-methoxyphenyl)-5(R)-methoxyhepthan-3-one(1),gingerenone A(2),3,4-dihydroxybenzaldehyde(3),p-hydroxybenzaldehyde(4),oresbiusin B(5),rosmarinic acid(6),bis(2-ethyloctyl)phthalate(7),cylindol B(8),2-(3',4'-dihydroxyphenyl)-1,3-benzodioxole-5-aldehyde(9),benzyl-β-D-glucoside(10)and 1H-indole-3-carboxaldehyde(11),based on the spectroscopic method.Compound 1 showed obvious inhibitory effects on Pseudomonas solanacearum and Clostridium perfringens,with the MIC value of 71.2μg/mL.Moreover,compound 2 inhibited potential inhibitory effects on Pseudomonas solanacearum,Clostridium perfringens and Bacillus subtilis with the MIC value of 77.6μg/mL.Conclusion Compound 1 is a new compound,named as salviacurcumin.Except for 3 and 6,all compounds are isolated from this plant for the first time.Accordingly,compounds 2,7,8 and 9 are isolated from Labiatae for the first time,5 is obtained from Salvia genus for the first time.Moreover,Compounds 1 and 2 exhibited potential antibacterial activity.
作者
梁会
赵玉敏
刘翰飞
娄华勇
潘卫东
LIANG Hui;ZHAO Yu-min;LIU Han-fei;LOU Hua-yong;PAN Wei-dong(The Second Affiliated Hospital,Guizhou University of Traditional Chinese Medicine,Guiyang 550001,China;Guizhou Medical University,State Key Laboratory of Functions and Applications of Medicinal Plants/School of Pharmaceutial Sciences,Guiyang 550014,China;Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;The Key Laboratory of Chemistry for Natural Product of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China)
出处
《中草药》
CAS
CSCD
北大核心
2022年第18期5593-5599,共7页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(32100322)
国家自然科学基金资助项目(U1812403)
国家自然科学基金资助项目(32060100)
贵州省科技计划项目(黔科合基础-ZK[2021] 534)。
