摘要
目的建立RP-HPLC法测定胃得安胶囊中23-乙酰泽泻醇B、辛弗林和厚朴酚3种成分的含量研究的分析方法。方法色谱条件:色谱柱为Waters Spherisorb ODS C_(18)柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-0.5%磷酸溶液(B)、梯度洗脱;检测器为二极管阵列检测器(DAD),采用仪器波长切换功能,分别设定23-乙酰泽泻醇B的检测波长为208 nm,辛弗林的为275 nm,厚朴酚的为294 nm;柱温为30℃;进样量为10μL;流速为1.0 mL/min;二极管阵列检测器定性、定量检测。结果23-乙酰泽泻醇B、辛弗林和厚朴酚分别在0.196~19.66μg/mL、0.299~29.94μg/mL和0.396~39.60μg/mL范围内线性关系良好,平均加标回收率分别为99.4%、99.6%和100.3%。结论所建立的方法具有检测准确度高、操作简便等优点,可用于胃得安胶囊供试品的质量控制。
Objective A RP-HPLC method was established for the determination of alisol B 23-acetate,synephrine and magnolol in Weidean capsules.Methods Waters Spherisorb ODS C_(18)(250 mm×4.6 mm,5μm)chromatographic column was used for separation.The mobile phase consisted of acetonitrile(A)-0.5%phosphoric acid solution(B)with gradient elution at the flow rate of 1.0 mL/min.The diode array detector(DAD)with a wavelength switching function is adopted for qualitative and quantitative analysis,and the detection wavelengths of three components were alisol B 23-acetate(208 nm),synephrine(275 nm),and magnolol(294 nm),respectively.The column temperature was 30℃and the injection volume was 10μL.Results The linearities of alisol B 23-acetate,synephrine and magnolol were in the range of 0.196-19.66μg/mL,0.299-29.94μg/mL and 0.396-39.60μg/mL,respectively.The average recoveries were 99.4%,99.6%and 100.3%,respectively.Conclusion The experimental results showed that the developed method had the advantages of high accuracy and simple operation,and could be used for the quality control of Weidean capsules.
作者
黄牧坤
王卫东
李明杰
HUANG Mukun;WANG Weidong;LI Mingjie(Department of Pharmacy,3201 Hospital,Hanzhong 723000,China)
出处
《广东药科大学学报》
CAS
2022年第5期66-69,共4页
Journal of Guangdong Pharmaceutical University
