摘要
随着现代生物工程技术快速发展,利用发酵技术提高草药提取物在食品和化妆品添加剂领域的生物活性已成为新趋势。在本研究中,以蘑菇酪氨酸酶活性和黑色素形成为评价指标,对5种益生菌的莲花提取物(LPE)发酵产物进行活性筛选。结果显示植物乳杆菌CGMCC 1.124发酵产物(FLPE)具有酪氨酸酶抑制活力;在A-MSH诱导的小鼠黑色素瘤细胞(B16F10)美白模型中,经50μg/mL FLPE处理的B16F10黑色素含量为模型对照组的86%±4.6%。为进一步阐明FLPE主要活性成分,利用HPLC,NMR和LC-MS对莲花提取物发酵前后产物中的主要黄酮类化合物进行定性定量分析。结构分析和生物活性评价结果表明,山奈酚-3-O-β-D-葡萄糖苷(49.8%,94.22 mg/g)和槲皮素-3-O-β-D-葡萄糖醛酸(13.6%)生物转化为两个生物活性苷元-槲皮素(14.4%,26.43 mg/g)和山奈酚(60.9%,70.76 mg/g)。在这些单体中,槲皮素通过调节酪氨酸酶活性、黑素发生相关酶(TYR,TRP-1和TRP-2)和小眼畸形相关转录调控因子(MITF)的表达抑制黑素形成。总体而言,利用植物乳杆菌CGMCC 1.124发酵提高了莲花提取物美白功效,转化生成的槲皮素是植物乳杆菌发酵产物抑制黑色素合成的物质基础之一。
Considering the growing evidence that microbial fermentation, as an optimum processing method, can improve the biological activity of herbal extracts in food and cosmetic additives fields. In recent years, owing to the unique aroma and pharmacological activity, lotus petals extract has been utilized in the preparation of cosmetic and food. However, none is available on the improvement of antimelanogenic activity in the microorganism-fermented lotus petals extract(LPE). Thus, in this study, it is hoped to enhance anti-melanogenic properties of lotus petals extract by using fermentation technology and clarify its mechanism of action. We screened five probiotics(L.plantarum CGMCC 1.124, L.fermentum DSMZ 20052, Bacillus subtilis CGMCC 1.943, B.natto CGMCC NO. 19463 and B.natto CGMCC NO. 19462) to ferment LPE with evaluating for their anti-melanogenic activity by mushroom tyrosinase and melanin formation inhibition assays, hoping to establish a new method to improve the anti-melanogenic activity of LPE. The results indicate that LPE fermented with L.plantarum CGMCC 1.124(FLPE), shows enhancement on anti-mushroom tyrosinase and anti-melanogenic activity in A-MSH-stimulated B16F10 melanocytes. In particular, compared with A-MSH incubation alone, co-incubation with 50 μg/m L FLPE reduces the melanin production to 86%±4.6%. To determine the main effectors of the anti-melanogenesis of FLPE, primary flavonoids from LPE and FLPE were isolated and determined by high performance liquid chromatography(HPLC), nuclear magnetic resonance(^(1)H NMR,C NMR) and liquid chromatography mass spectrometry(LC-MS) analyses. The results of structure analysis and bioactivity evaluation show that flavonoid glycosides kaempferol-3-O-β-D-glucopyranoside(49.8%, 94.22 mg/g) and quercetin-3-O-β-D-glucuronide(13.6%) are bio-transformed to two bioactive aglycones, quercetin(14.4%, 26.43 mg/g) and kaempferol(60.9%, 70.76 mg/g). Among these monomers, quercetin and kaempferol decrease the mushroom tyrosinase activity, but only quercetin shows anti-melanogenic activity by suppressing tyrosinase activity in B16F10 cells, regulating the m RNA expressions of melanogenesis-related enzymes(TYR, TRP-1 and TRP-2) and microphthalmia-associated transcription factor(MITF) and reducing the protein contents of TYR, TRP-1 and MITF in a dose-dependent manner. These data suggest that FLPE has a potential for skin whitening with quercetin as a functional compound. These results maybe contribute to developing healthy food and cosmetic, and expand the range of application of lotus petals.
作者
赵胜男
郭苗苗
蔡孟浩
洪民华
吕智
安法梁
Shengnan Zhao;Miaomiao Guo;Menghao Cai;Minhua Hong;Zhi Lv;Faliang An(University of Bioengineering,East China University of Science and Technology,Shanghai 200237,China;Key Laboratory of Cosmetic,Beijing Technology and Business University,Beijing 100048,China;Technology Center,Shanghai Inoherb Co.,Ltd.,Shanghai 200083,China)
出处
《日用化学工业（中英文）》
CAS
北大核心
2022年第10期1029-1039,共11页
China Surfactant Detergent & Cosmetics
基金
上海市宝山区科学技术委员会科技创新专项资金项目(No.17-C-1)
中国轻工业化妆品重点实验室(北京工商大学)开放研究基金项目(KLC-2019-ZD2)。
关键词
莲花
发酵
抗黑色素生成
植物乳杆菌
槲皮素
lotus petals
fermentation
anti-melanogenesis
Lactobacillus plantarum
quercetin
