摘要
目的分析新型冠状病毒肺炎(COVID-19)康复患者与健康人群新型冠状病毒疫苗接种者血清抗体水平差异。方法收集2020年1月—2020年10月四川省治愈出院并于四川省人民医院随访的COVID-19康复者12例作为康复组,43例接种2剂新冠疫苗满6个月的健康人群作为免疫组,51例接种新冠疫苗加强针(第三针)28~35 d的健康人群作为加强组,3组人群定量检测免疫指标、SARS-CoV-2特异性IgG抗体和中和抗体。结果康复组与免疫组特异性IgG抗体阳性率均为100%,康复者特异性IgG抗体均值为43.44 AU/mL,明显高于免疫组特异性IgG抗体均值25.02 AU/mL(t=-3.077,P<0.05);康复组、免疫组、加强组中和抗体阳性率分别为83.33%、32.56%、100.00%,中和抗体均值分别为29.03、4.63、32.03 AU/mL,康复组出院8~14个月后体内平均中和抗体水平是免疫组的6.3倍,加强组平均中和抗体水平是免疫组6.9倍。加强组中男性中和抗体均值为61.68 AU/mL,明显高于女性中和抗体均值26.13 AU/mL(P<0.05),而BMI分组、年龄分组在各组内比较差异无统计学意义(P>0.05)。在免疫功能方面,康复组中CD4+T淋巴细胞计数均值为415.00 cells/μL,明显低于免疫组CD4+T淋巴细胞计数均值590.00 cells/μL(P<0.05),而免疫球蛋白A、G、M及补体3、补体4在康复组及免疫组中比较差异无统计学意义(P>0.05)。结论COVID-19患者在疾病发展过程中可产生IgG抗体和中和抗体,且高于接种2剂新冠疫苗者;新冠疫苗加强针能诱发更高的抗体水平,且男性中和抗体水平高于女性。
ObjectiveTo analyze the differences in serum antibody levels between COVID-19 recovered patients and healthy people who were after the COVID-19 vaccination. Methods12 patients with COVID-19 who were cured and discharged from hospitals in Sichuan Province and followed up in our hospital from January 2020 to October 2020 were collected as the rehabilitation group. 43 healthy persons who had been vaccinated with 2 doses of COVID-19 vaccines for 6months were used as immunization group. 51 healthy persons who inoculated with the booster injection(the third injection) for28-35 days were used as the booster group. The immune indicators, SARS-CoV-2 IgG antibody and neutralizing antibody were detected quantitatively in the three groups.ResultsThe positive rate of antibody IgG in both the rehabilitation group and the immunization group was 100%. The average specific IgG antibody of the rehabilitation group was 43.44 AU/mL, which was significantly higher than that of the immunization group, which was 25.02 AU/mL(P<0.05);The positive rates of neutralizing antibodies in the rehabilitation group, immunization group, and booster group were 83.33%, 32.56%, and 100.00%respectively, and the average neutralizing antibody level were 29.03, 4.63, and 32.03 AU/mL, respectively. The average neutralizing antibody level in the rehabilitation group after 8-14 months of discharge was 6.5 times higher than that in the immunization group. The average neutralizing antibody level in the booster group was 6.9 times that of the immunization group.In the booster group, the mean neutralizing antibody of males was 61.68 AU/mL, which was significantly higher than that of females, which was 26.13 AU/mL(P<0.05). There was no significant difference in BMI group and age group among the groups(both P>0.05). In terms of immune function, the mean CD4T lymphocyte count in the rehabilitation group was 415.00 cells/μL,which was significantly lower than the mean CD4T lymphocyte count in the immunization group, 590.00 cells/μL(P<0.05).However, there was no significant difference in immunoglobulin A, G, M, complement 3 and complement 4 between the rehabilitation group and the immunization group(P>0.05).ConclusionsCOVID-19 patients can produce IgG antibodies and neutralizing antibodies during the development of the disease, and higher than those who received 2 doses of the COVID-19vaccines. The COVID-19 vaccine booster can induce higher antibody level, and the level of neutralizing antibody in male is higher than that in female.
作者
景雪
罗婷婷
庹琳
杨兴祥
JING Xue;LUO Ting-ting;TUO Lin;YANG Xing-xiang(Department of infection,Santai County People's Hospital,Mianyang 621100,Sichuan,China;Department of Infection,Sichuan Academy of Medical Science&Sichuan Provincial People's Hospital,Chengdu,Sichuan 610072,China)
出处
《中国热带医学》
CAS
2022年第8期776-779,790,共5页
China Tropical Medicine
基金
2020年四川省卫生健康委员会医学重大创新项目(No.20ZDCX002)。
