摘要
目的观察大黄素对高热量饲料喂养所致大鼠非酒精性脂肪肝(NAFLD)的治疗作用及对肝组织11β-羟基类固醇脱氢酶1(11β-hydroxysteroid dehydrogenase type 1,11β-HSDI)表达的影响。方法雄性Wistar大鼠40只,随机分为对照组、模型组、大黄素低剂量组(E1组)、大黄素高剂量组(E2组),每组10只。对照组给予普通饲料,其余3组喂以自制高脂饲料,从第9周开始治疗组给予大黄素干预治疗,第14周末实验结束,进行相关指标检测。结果与对照组比较,模型组大鼠出现了明显的NAFLD病变,且肝组织11β-HSD1表达增加;与模型组比较,大黄素治疗可以降低肝脏指数,降低肝功能指标ALT、AST,降低血脂及肝脏脂质沉积,减轻肝组织脂肪变性、炎性反应及纤维化,同时显著降低肝组织11β-HSD1基因和蛋白表达量。且以上治疗作用呈剂量依赖性。结论大黄素对NAFLD有保护作用,其作用机制可能与抑制11β-HSD1活性有关。
Objective To observe the effect of emodin on nonalcoholic fatty liver disease(NAFLD)and the expression of 11β-HSD1 in liver.To investigate the expression changes of 11β-hydroxysteroid dehydrogenase type 1(11β-HSD1)in the liver of rats with nonalcoholic fatty liver(NAFLD)and the intervention effect of emodin.Method 40 male Wistar rats were randomly divided into four groups:control,model,low-dosage emodin treatment(E1)and high-dosage emodin treatment(E2)groups.The control group was feed with normal diet,the model and treatment group were feed with high-fat diet.The treatment group was given emodin from the 9th week,and the experiment was completed at the end of the 14th week.Result Compared with the control group,rats in the model group had significant NAFLD,and the expression of 11β-HSD1 in liver was increased.Compared with the model group,emodin treatment could reduce liver index,blood lipid and liver lipid deposition,improve liver function and pathological changes,and significantly inhibit the expression of 11β-HSD1 gene and protein in liver tissue.All emodin treatment effects were dose-dependent.Conclusion Emodin has a significant protective effect on NAFLD,which maybe related to the inhibition of the 11β-HSD1 activity.
作者
孙红爽
李鹏霖
刘永双
乜春城
种宝贵
SUN Hongshuang;LI Penglin;LIU Yongshuang;NIE Chuncheng;CHONG Baogui(Department of Pharmacy,Harrison International Peace Hospital,Hengshui 053000,China)
出处
《实验动物科学》
2022年第3期22-26,32,共6页
Laboratory Animal Science
基金
河北省2021年度医学科学研究课题(20211184)。
